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Thromb Haemost 1994; 72(06): 912-918
DOI: 10.1055/s-0038-1648983
Original Article
Schattauer GmbH Stuttgart

Effects of Magnesium on Platelet Aggregation and Adhesion

Magnesium Modulates Surface Expression of Glycoproteins on Platelets In Vitro and Ex Vivo
M Gawaz
1   The First Medical Department and Institute of Anatomy, Technical University, Munich, Germany
,
I Ott
1   The First Medical Department and Institute of Anatomy, Technical University, Munich, Germany
,
A J Reininger
2   Institute of Anatomy, Technical University, Munich, Germany
,
F-J Neumann
1   The First Medical Department and Institute of Anatomy, Technical University, Munich, Germany
› Author Affiliations
Further Information

Publication History

Received 07 January 1994

Accepted after resubmission 30 August 1994

Publication Date:
06 July 2018 (online)

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Summary

Magnesium deficiency and its association with platelet hyperreactivity has been well recognised in a variety of diseases including myocardial infarction, preeclampsia, and diabetes. In order to investigate potential effects of intravenous Mg2+ supplementation, platelet function was studied by measurements of in vitro bleeding time (BT) and of fibrinogen (Fg)-mediated aggregation of washed platelets. In addition, the effect of Mg2+ on platelet adhesion onto immobilised Fg, on Fg binding to activated platelets, and on surface expression of GMP-140 or GP53 was evaluated. Mg2+(4 mM) prolonged in vitro BT by 30% and inhibited Fg-mediated aggregation significantly, independent of the agonist used to initiate platelet aggregation (ADP, collagen, epinephrine, thrombin, phorbol ester). Adhesion of resting platelets to immobilised Fg was reduced by 50% in the presence of 2 mM Mg2+. Moreover, Mg2+ reduced Fg binding to ADP- or collagen-stimulated platelets as well as surface expression of GMP-140 with an IC50 of approximately 3 mM. Intravenous administration of Mg2+ to healthy volunteers inhibited both ADP-induced platelet aggregation (p <0.05) by 40% and binding of Fg or surface expression of GMP-140 by 30% (p <0.05). Thus, pharmacological concentrations of Mg2+ effectively inhibit platelet function in vitro and ex vivo.

 

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