Effect of Plasma and Platelet Concentrate Infusions on Factor VIII, Platelet Adhesiveness, and Bleeding Time in Bleeder Swine

C. N Cornell; R. G Cooper; M. E Muhrer; S Garb

doi:10.1055/s-0038-1649026

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1972; 28(03): 431-439
DOI: 10.1055/s-0038-1649026

Original Article

Schattauer GmbH

Effect of Plasma and Platelet Concentrate Infusions on Factor VIII, Platelet Adhesiveness, and Bleeding Time in Bleeder Swine

Authors

C. N Cornell

¹Departments of Agricultural Chemistry and Pharmacology of the University of Missouri, Columbia, Missouri 65201
R. G Cooper

¹Departments of Agricultural Chemistry and Pharmacology of the University of Missouri, Columbia, Missouri 65201
M. E Muhrer

¹Departments of Agricultural Chemistry and Pharmacology of the University of Missouri, Columbia, Missouri 65201
S Garb

¹Departments of Agricultural Chemistry and Pharmacology of the University of Missouri, Columbia, Missouri 65201

Abstract

Summary

Bleeder swine are characterized by a prolonged bleeding time, decreased factor VIII levels or reduced platelet adhesiveness or both, and a factor VIII response to infusion of plasma or serum similar to that described in von Willebrand’s disease. In this study the factor VIII response of bleeder swine infused with fresh non-glass-contact pig plasma was similar to that reported in previous experiments after infusion of plasma that was frozen and stored for a variable period of time before use or was not free of glass contact. There was no beneficial effect on platelet adhesiveness. Following platelet concentrate infusion, there was no sustained normalization of platelet adhesiveness, although elevations into the normal range occurred at varying intervals after platelet infusion in two of three pigs investigated. Infusion of factor VIII-deficient plasma from a canine hemophiliac elicited a factor VIII response similar to that described in von Willebrand’s disease patients infused with plasma from factor VIII-deficient human hemophiliacs. In none of these experiments was there any apparent correction of the bleeding time.

Volltext

Referenzen

References
1 Biggs R, and Matthews J. M. 1963; The treatment of haemorrhage in von Willebrand’s disease and the blood level of factor VIII (AHG). British Journal of Haematology 9: 203.
2 Bogart R, and Muhrer M. E. 1942; The inheritance of a hemophilia-like condition in swine. Journal of Heredity 33: 59.
3 Borchgrevink C. F, and Owren P. A. 1961; The hemostatic effect of normal platelets in hemophilia and factor V deficiency. Acta Medica Scandinavica 170: 375.
4 Chan J. Y. S, Owen Jr. C. A., Bowie E. J. W, Didishiem P, Thompson Jr. J. H., Muhrer M. E, and Zollman P. E. 1968; von Willebrand’s disease “stimulating factor” in porcine plasma. American Journal of Physiology 214: 1219.
5 Cooper R. G, Cornell C. N, Muhrer M. E, and Garb S. 1969; Platelet adhesion in bleeder swine measured by glass bead column. Texas Reports on Biology and Medicine 27: 955.
6 Cooper R. G, Hale B. G, Cornell C. N, and Muhrer M. E. 1971; Blood platelet size distribution in the Missouri bleeder swine. Life Sciences 10: 1247.
7 Cornell C. N, Cooper R. G, Kahn R. A, and Garb S. 1969; Platelet adhesiveness in normal and bleeder swine as measured in a Celite system. American Journal of Physiology 216: 1170.
8 Cornell C. N, Cooper R. G, Muhrer M. E, and Garb S. 1972; Splenectomy and factor VIII response in bleeder swine. American Journal of Physiology 222: 1610.
9 van Creveld S, and Mochtar I. A. 1960; von Willebrand’s disease, a plasma deficiency cause of the prolonged bleeding time. Annales Paediatrici 194: 37.
10 Cornu P, Larrieu M. J, Caen J, and Bernard J. 1963; Transfusion studies in von Willebrand’s disease : effect on bleeding time and factor VIII. British Journal of Haematology 9: 189.
11 Dormandy K. 1968; Cryoprecipitate and the use of the plastic bag system in the management of haemophilia and other coagulation disorders. Proceedings of the Royal Society of Medicine 61: 595.
12 Fantl P, and Sawers R. J. 1963; Stimulation of factor VIII (antihemophilic) activity by transfused serum. Nature 200: 1214.
13 Flatow Jr. F. A., and Freireich E. J. 1966; The increased effectiveness of platelet concentrates prepared in acidified plasma. Blood 27: 449.
14 Kahn R. A, Cooper R. G, Cornell C. N, and Muhrer M. E. 1970; Electron microscopy of bleeder swine platelets. American Journal of Veterinary Research 31: 679.
15 Langdell R. D, Wagner R. H, and Brinkhous K. M. 1964. Estimation of antihemophilic activity by the partial thromboplastin time technique. In: Tocantins L. M, and Kazal L. A. (eds.) Blood, Coagulation, Hemorrhage and Thrombosis. Grune and Stratton; New York.: 107.
16 Larrieu M. J, Caen J. P, Meyer D. O, Vainer H, Sultan Y, and Bernard J. 1968; Congenital bleeding disorders with long bleeding time and normal platelet count. II. von Willebrand’s disease. American Journal of Medicine 45: 354.
17 Mertz E. T. 1942; The anomaly of a normal Duke’s and a very prolonged saline bleeding time in swine suffering from an inherited bleeding disease. American Journal of Physiology 136: 360.
18 Meyer D, Larrieu M. J, Maroteaux P, and Caen J. P. 1967; Biological findings in von Willebrand’s pedigrees : implications for inheritance. Journal of Clinical Pathology 20: 190.
19 Muhrer M. E, Lechler E, Cornell C. N, and Kirkland J. 1965; Antihemophilic factor levels in bleeder swine following infusions of plasma and serum. American Journal of Physiology 208: 508.
20 Nilsson I. M, Blomback M, and Blomback B. 1959; v. Willebrand’s disease in Sweden. Its pathogenesis and treatment. Acta Medica Scandinavica 164: 263.
21 Perkins H. 1967; Correction of the hemostatic defects in von Willebrand’s disease. Blood 30: 375.
22 Salzman E. W. 1963; Measurement of platelet adhesiveness. Journal of Laboratory and Clinical Medicine 62: 724.
23 Stormorken H. 1967. The defect in von Willebrand’s disease. In: Johnson S.A, and Seegers W. H. (eds.) Physiology of Hemostasis and Thrombosis. Charles C. Thomas; Springfield, Illinois,: 179.
24 Strauss H. S, and Bloom G. E. 1965; von Willebrand’s disease: use of a platelet adhesiveness test in diagnosis and family investigation. New England Journal of Medicine 273: 171.
25 Sutor A. H, Bowie E. J. W, and Owen Jr. C. A.. 1971; A new method of demonstrating the effect of therapy in von Willebrand’s disease. Mayo Clinic Proceedings 46: 345.
26 Weiss H. J. 1962; The use of plasma and plasma fractions in the treatment of a patient with von Willebrand’s disease. Vox Sanguinis 7: 267.
27 White Jr. J., Yunis E, Colliander M, and Krivit W. 1963; Prolonged bleeding time in a patient with plasma thromboplastin antecedent deficiency: observations on correction of the bleeding time by platelet transfusion. Journal of Pediatrics 63: 1081.