Influence of Factor VII on the Assay of Factor VIII by the One Stage Method Using Wettable Surface

Raúl Altman; Jorge Rouvier; Carlos Reussi; with the technical assistance of Silvia M. Taboada

doi:10.1055/s-0038-1649029

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1972; 28(03): 457-463
DOI: 10.1055/s-0038-1649029

Original Article

Schattauer GmbH

Influence of Factor VII on the Assay of Factor VIII by the One Stage Method Using Wettable Surface

Raúl Altman M. D.

¹Section on Hemostasis and Thrombosis, Department of Internal Medicine and Department of Docencia e Investigación, U.A.M. I. Rivadavia-Peralta Ramos, Buenos Aires, Argentina

,

Jorge Rouvier M. D.

¹Section on Hemostasis and Thrombosis, Department of Internal Medicine and Department of Docencia e Investigación, U.A.M. I. Rivadavia-Peralta Ramos, Buenos Aires, Argentina

,

Carlos Reussi M. D.

¹Section on Hemostasis and Thrombosis, Department of Internal Medicine and Department of Docencia e Investigación, U.A.M. I. Rivadavia-Peralta Ramos, Buenos Aires, Argentina

,

with the technical assistance of Silvia M. Taboada

› Author Affiliations

Abstract

Summary

The influence of factor VII activated by Contact Product (i. e. the product of the reaction between factor XII and XI) on the factor VIII determination by the partial thromboplastin time with kaolin is described. There is an important decrease in clotting time when, with a constant amount of factor VIII, the concentration of factor VII is increased. This difference is unimportant from a practical point of view; but this does not distract from the possibility that the minute amount of thrombin generated by the XII-VII pathway may be of great practical interest, because it may be capable of activation factors V and VIII as well as influencing platelet metabolism and thus induce hemostasis, especially when the intrinsic pathway is blocked.

Full Text

References

References
1 Altman R, and Hemker H. C. 1967; Contact activation in the extrinsic blood clotting system. Thrombosis et Diathesis Haemorrhagica 18: 525.
2 Casillas G, Simonetti C, and Pavlovsky A. 1969; Chromatographic behaviour of clotting factors. British Journal of Haematology 16: 363.
3 Hemker H. C, Hemker P. W, and Loeliger E. A. 1965; Kinetic aspects of the interaction of blood clotting enzymes. I. Derivation of Basic Formulas. Thrombosis et Diathesis Haemorrhagica 13: 155.
4 Proctor R. R, and Rapaport S. I. 1958; The partial thromboplastin time with kaolin. American Journal of Clinical Pathology 29: 104.
5 Rapaport S. I, Hjort P. F, and Patch M. J. 1965; Further evidence that thrombin activation of factor VIII is an essential step in intrinsic clotting. Scandinavian Journal of Clinical and Laboratory Investigation 17 (84) 88.