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Thromb Haemost 1972; 28(02): 206-212
DOI: 10.1055/s-0038-1649056
Original Article
Schattauer GmbH

Differing Procoagulant Activities in the Extracted Lipids of High and Low Density Platelet Plasma Membranes

A. J Barber
1   American National Red Cross Blood Research Laboratory Bethesda, Maryland 20014, U.S.A.
,
D. C Triantaphyllopoulos
1   American National Red Cross Blood Research Laboratory Bethesda, Maryland 20014, U.S.A.
,
G. A Jamieson
1   American National Red Cross Blood Research Laboratory Bethesda, Maryland 20014, U.S.A.
› Author Affiliations
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Publication History

Publication Date:
29 June 2018 (online)

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Summary

Platelet factor 3 activity has been examined in the high (d,1.120) and low (d,1.090) density platelet membranes obtained by the glycerol-lysis technique, and in the lipid fractions extracted from them. The following observations were made : procoagulant activity, as determined by the thromboplastin generation test, was equal in the two types of membrane and was higher than in the intracellular fractions ; the lipid fraction extracted from the low density membranes had a higher activity than that from the high density membranes; at high concentrations this extracted phospholipid had a mild anticoagulant effect. These results demonstrate functional differences between the two types of platelet membrane and favor a plasma membrane origin for platelet factor 3.

 

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