Studies on Fibrin Formation and Effects of Dextran

T. Z Muzaffar; G. G Youngson; W. A. J Bryce; D. P Dhall

doi:10.1055/s-0038-1649061

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1972; 28(02): 244-256
DOI: 10.1055/s-0038-1649061

Original Article

Schattauer GmbH

Studies on Fibrin Formation and Effects of Dextran

Authors

T. Z Muzaffar

¹Departments of Surgery and Chemistry, The University of Aberdeen, Aberdeen, Scotland
G. G Youngson

¹Departments of Surgery and Chemistry, The University of Aberdeen, Aberdeen, Scotland
W. A. J Bryce

¹Departments of Surgery and Chemistry, The University of Aberdeen, Aberdeen, Scotland
D. P Dhall

¹Departments of Surgery and Chemistry, The University of Aberdeen, Aberdeen, Scotland

Abstract

Summary

In this study highly reproducible turbidimetric techniques are used to investigate clotting of human platelet-poor plasma and purified human fibrinogen solutions by thrombin. Three phases are clearly distinguishable turbidimetrically during clotting. All the three phases are altered by dextran. This effect of dextran is not mediated by any action on the enzymatic activity of thrombin. Further, it is independent of the molecular weight of dextran, but is found related to the final dextran, thrombin and fibrinogen concentrations. Evidence is presented to show that dextran accelerates the polymerisation of fibrin monomer. However, it seems clear that dextran has an additional action quite apart from this effect. These actions of dextran are discussed in relation to kinetics of clot formation.

Full Text

References

References
1 Abildgaard W. 1965; N-Terminal analysis during coagulation of purified human fibrinogen, fraction I and plasma. Scandinavian Journal of Clinical and Laboratory Investigation 17: 529.
2 Abildgaard W. 1966; Acceleration of fibrin polymerisation by dextran and ficoll. Interaction with calcium and plasma proteins. Scandinavian Journal of Clinical and Laboratory Investigation 18: 518.
3 Blombäck B. 1967. Fibrinogen to Fibrin Transformation. In: Seegers W. H. (ed.) Blood Clotting Enzymology. Academic Press; New York and London: 143.
4 Blombäck B, Blombäck M, and Nilsson I. M. 1957; Coagulation studies on “Reptilase”, an extract of the venom from Bothrops jararaca. Thrombosis et Diathesis Haemorrhagica 1: 76.
5 Blombäck B, and Laurent T. C. 1958; N. Terminal and light scattering studies on fibrinogen and its transformation to fibrin. Arkie far Kemi 12: 137.
6 Bonnar J, and Walsh J. 1972; Prevention of thrombosis after pelvic surgery by British dextran 70. Lancet I: 614.
7 Burstein M. 1952; Substances fibrinoplastiques et antifibrinoplastiques. Revue Hematologique 7: 587.
8 Bydgeman S. 1968; Experimental studies on the antithrombotic effect of dextran. Acta chirurgica Scandinavica Supplement 387: 44.
9 Dhall D. P, Bryce W. A. J. 1970 Effect of dextran on kinetics of fibrin formation. 6th European Conference on Microcirculation, Aalborg. p. 375.
10 Eagle H, and Harris T. N. 1937; Studies in blood coagulation. V. The coagulation of blood by proteolytic enzymes (Trypsin, Papain). Journal of general Physiology 20: 543.
11 Edsall J. T, and Lever W. F. 1951; Effects of ions and neutral molecules on fibrin clotting. The Journal of Biological Chemistry 191: 735.
12 Ferry J. D, and Morrison P. R. 1947; Preparation and properties of serum and plasma protein. VIII. The conversion of human fibrinogen to fibrin under various conditions. Journal of the American Chemical Society 69: 388.
13 Ferry J. D, and Shulman S. 1949; The conversion of fibrinogen to fibrin. I. Influence of hydroxyl compounds on clotting time and clot opacity. Journal of the American Chemical Society 71: 3198.
14 Heller W. J. 1957; Theoretical investigations of the light scattering by colloidal spheres. II. Accurate interpolation of theoretical turbidity data. Journal of Chemical Physics 26: 920.
15 Jacobeus IT. 1957; Studies on effect of dextran on the coagulation of blood. Acta Medica Scandinavica Supplement 332: 41.
16 Lambie J, Barber D, Dhall D. P, and Matheson N. A. 1970; Dextran 70 in the prophylaxis of post-operative venous thrombosis. A controlled trial. British Medical Journal 2: 144.
17 Laurell A. B. 1951; Influence of dextran on conversion of fibrinogen to fibrin. Scandinavian Journal of Clinical and Laboratory Investigation 3: 262.
18 McFarlane A. S. 1963; In vivo behaviour of I¹³¹ fibrinogen. Journal of Clinical Investigation 42: 346.
19 Mihalyi E. 1950; Properties of fibrin dissolved in urea solutions. Acta Chemica Scandinavica 4: 344.
20 Muzaffar T. Z, Stalker A. L, Bryce W. A. J, and Dhall D. P. 1972 Structural alterations in fibrin clots with dextran. Thrombosis et Diathesis Haemorrhagica, this edition.
21 Seegers W. H. 1952; A new plasma antithrombin reaction. Archives of Biochemistry and Biophysics 36: 484.
22 Sherry S, and Troll W. 1954; The action of thrombin on synthetic substances. The Journal of Biological Chemistry 208: 95.