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Thromb Haemost 1972; 28(02): 268-279
DOI: 10.1055/s-0038-1649063
Original Article
Schattauer GmbH

Parallel Determinations of FDP and Fibrin Monomers with Various Methods

U Hedner
1   Coagulation Laboratory, Allmänna Sjukhuset, Malmö, Sweden
,
I. M Nilsson
1   Coagulation Laboratory, Allmänna Sjukhuset, Malmö, Sweden
› Institutsangaben
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Publikationsdatum:
29. Juni 2018 (online)

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Summary

The following tests were performed in 305 persons with one or other of the following conditions: malignant disease, postoperative complications, sepsis or multiple fractures, liver disease, pregnancy, the puerperium, renal disease, dysproteinaemia and different blood disorders : 1. fibrinogen degradation products (FDP) with Niléhn’s immunochemical method; 2. the thrombin time; 3. the Reptilase time; 4. the ethanol gelation test; 5. the protamine sulphate precipitation test. It was found that a prolonged thrombin time or Reptilase time in a given case was not necessarily a sign of the presence of FDP. Thus, neither determination of the thrombin time nor of the Reptilase time can substitute for specific determination of FDP. A positive ethanol gelation test was found in only 5 out of 17 patients with a low platelet count, low P & P test, low factor V, low fibrinogen and clinical signs of disseminated intravascular coagulation such as shock, disturbance of the periphery circulation, oliguria or anuria. No association was found between a positive ethanol gelation test and the presence of FDP. The protamine sulphate precipitation test was positive in only one of the 305 patients.

 

  • References

  • 1 Alkjaersig N, Fletcher A. P, and Sherry S. 1962; Pathogenesis of the coagulation defect developing during pathological plasma proteolytic (“fibrinolytic”) states. II. The significance, mechanism and consequences of defective fibrin polymerization. J. Clinical Investigation 41: 917.
    CrossrefPubMedSuche in Google Scholar
  • 2 Hedner Nilsson, Allington M. J. 1971; The latex flocculation inhibition test for the detection of fibrin(ogen) degradation products. Scandinavian Journal of Haematology Suppl. 13: 9 115, 389.
    PubMedSuche in Google Scholar
  • 3 Arnesen H. 1971 Fibrinogen determinations in the presence of fibrinogen degradation products. II. Congress of the International Society on Thrombosis and Haemostasis, Oslo, Norway July 12 th-16th.
    PubMedSuche in Google Scholar
  • 4 Breen F. A, and Tullis J. L. 1968; Ethanol gelation: a rapid screening test for intravascular coagulation. Annals Internal Medicine 69: 1197.
    CrossrefPubMedSuche in Google Scholar
  • 5 Deykin D. 1970; The clinical challenge of disseminated intravascular coagulation. New England Journal of Medicine 283: 636.
    CrossrefPubMedSuche in Google Scholar
  • 6 Donati M. B, Vermylen J, Verstraete M. 1971; Fibrinogen degradation in vivo : Effect on the reptilase time and the thrombin time. Scandinavian Journal of Haematology Suppl. 13: 259.
    PubMedSuche in Google Scholar
  • 7 Godal H. C, and Abildgaard U. 1966; Gelation of soluble fibrin in plasma by ethanol. Scandinavian Journal of Haematology 3: 342.
    PubMedSuche in Google Scholar
  • 8 Godal H. C, Abildgaard U, and Kierulf P. 1971; Ethanol gelation and fibrin monomers in plasma. Scandinavian Journal of Haematology Suppl 13: 189.
    PubMedSuche in Google Scholar
  • 9 Hallen A, and Nilsson I. M. 1964; Coagulation studies in liver disease. Thrombosis et Diathesis Haemorrhagica 11: 51.
    PubMedSuche in Google Scholar
  • 10 Hedner U, and Nilsson I. M. 1971; Clinical experience with determination of fibrinogen degradation products. Acta medica Scandinavica 189: 471.
    PubMedSuche in Google Scholar
  • 11 Ingram G. I. C. 1955; Variations in the reaction between thrombin and fibrinogen and the effect on the prothrombin time. Journal of Clinical Pathology 8: 318.
    CrossrefPubMedSuche in Google Scholar
  • 12 Jim R. T. S. 1957; A study of the plasma thrombin time. Journal of Laboratory and Clinical Medicine 50: 45.
    PubMedSuche in Google Scholar
  • 13 Komp D. M, and Sparrow A. W. 1970; Quantitation of secondary fibrinolysis in cyanotic heart disease. Pediatrics 77: 679.
    PubMedSuche in Google Scholar
  • 14 Latallo Z. S, Wegrzynowicz Z, Budzynski A. Z, and Kopeć M. 1971; Effect of protamine sulphate on the solubility of fibrinogen, its derivatives and other plasma proteins. Scandinavian Journal of Haematology, Suppl 13: 151.
    PubMedSuche in Google Scholar
  • 15 Latallo Z. S, Wegrzynowicz Z, Teisseyre E, and Kopeć M. 1970; Simple and rapid evaluation of the intravascular coagulation and fibrinolytic status by application of protamine sulphate and Reptilase. Scand. Haematol. Suppl 13: 387.
    PubMedSuche in Google Scholar
  • 16 Marder V. J, Larrieu M.-J, and Shulman N. R. 1966; Molecular characteristics and anticoagulant activity of intermediate products of fibrinogenolysis. Blood 28: 985.
    PubMedSuche in Google Scholar
  • 17 Marder V. J, Shulman N. R, and Carroll W. R. 1967; The importance of intermediate degradation procuts of fibrinogen in fibrinolytic hemorrhages. Transactions of the Association of American Physicians 80: 156.
    PubMedSuche in Google Scholar
  • 18 Margolis C. Z. 1971; Ethanol gelation test. New England Journal of Medicine 284: 53.
    PubMedSuche in Google Scholar
  • 19 Melliger E. J. 1970; Detection of fibrinogen degradation products by use of antibody coated latex particles. Thrombosis et Diathesis Haemorrhagica 23: 211.
    PubMedSuche in Google Scholar
  • 20 Merskey C, Kleiner G. J, and Johnson A. J. 1966; Quantitative estimation of split products of fibrinogen in human serum. Relation to diagnosis and treatment. Blood 28: 1.
    PubMedSuche in Google Scholar
  • 21 Niewiarowski S, and Kowalski E. 1957; Formation of an antithrombinlike anticoagulant during proteolysis of fibrinogen. Bulletin de l’Académie de polonaise des Sciences 5: 169.
    PubMedSuche in Google Scholar
  • 22 Niléhn J.-E. 1967; Separation and estimation of “split products” of fibrinogen and fibrin in human serum. Thrombosis et Diathesis Haemorrhagica 18: 487.
    PubMedSuche in Google Scholar
  • 23 Niléhn J.-E. 1967; Influence of split products of fibrinogen on results of blood coagulation tests and platelet adhesiveness. Scandinavian Journal of Haematology 4: 430.
    PubMedSuche in Google Scholar
  • 24 Niléhn J.-E, and Nilsson I. M. 1964; Demonstration of fibrinolytic split products in human serum by an immunological method in spontaneous and induced fibrinolytic states. Scandinavian Journal of Haematology 1: 313.
    PubMedSuche in Google Scholar
  • 25 Nilsson I. M, Blombäck M, and von Francken I. 1957; On an inherited autosomal haemorrhagic diathesis with antihemophilic globulin (AHG) deficiency and prolonged bleeding time. Acta medica Scandinavica 159: 35.
    PubMedSuche in Google Scholar
  • 26 Nilsson I. M, Blombäck M, and Ramgren O. 1961; Haemophilia in Sweden. I. Coagulation studies. Acta medica Scandinavica 170: 665.
    PubMedSuche in Google Scholar
  • 27 Nillson I. M, and Olow B. 1962; Determination of fibrinogen and fibrinogenolytic activity. Thrombosis et Diathesis Haemorrhagica 8: 297.
    PubMedSuche in Google Scholar
  • 28 Paraskevas M, Nilsson I. M, and Martinsson G. 1962; A method for determining serum inhibitors of plasminogen activation. Scandinavian Journal of Clinical and Laboratory Investigation 14: 138.
    CrossrefPubMedSuche in Google Scholar
  • 29 Perry S. 1963; Coagulation factors in patients with plasma protein disorders. Journal of Laboratory and Clinical Medicine 61: 411.
    PubMedSuche in Google Scholar
  • 30 Seaman A. J. 1970; The recognition of intravascular clotting: the plasma protamine paracoagulation test. Archives of Internal Medicine 125: 106.
    CrossrefPubMedSuche in Google Scholar
  • 31 Straub P. W. 1971 The Reptilase time in presence of FDP. II. Congress of the International Society on Thrombosis and Haemostasis, Oslo, Norway July 12th–16h, 1971.
    PubMedSuche in Google Scholar