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Thromb Haemost 1973; 30(02): 299-306
DOI: 10.1055/s-0038-1649078
Original Article
Schattauer GmbH

Role of Platelet Factor 3 in the Hypercoagulability Induced by Pregnancy and Oral Contraceptives[*]

S Renaud   **
1   Laboratory of Experimental Pathology, Montreal Heart Institute and Department of Pathology, University of Montreal
,
P Gautheron   **
1   Laboratory of Experimental Pathology, Montreal Heart Institute and Department of Pathology, University of Montreal
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Publikationsverlauf

Received for publication 10. Januar 1973

Accepted for publication 07. Juni 1973

Publikationsdatum:
30. Juni 2018 (online)

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Summary

As evaluated in a recording “coagulometer” the Stypven time of 16 to 19 days pregnant Holtzman rats was shorter than that of the controls solely in platelet-rich plasma but not in platelet-poor plasma collected in siliconized material when most of the platelets were removed by slow speed centrifugation (1000 G × 20 minutes). If the same blood was centrifuged at a higher speed (2000 G) the Stypven time was considerably shorter in the pregnant rats even if no platelets were left indicating that platelet factor 3 had been released in plasma during centrifugation.

Identical results were obtained in 10 women taking oral contraceptives as compared to 10 controls. Depending on whether blood was collected in siliconized glassware or not, and final determination performed in plastic or glass, the cephalin clotting time of oral contraceptives treated women was found to be longer, equal or shorter than this of the controls. When it was shorter, a comparable reduction in the Stypven time indicated that the shortening was probably due to “release” of platelet factor 3 in the platelet-poor plasma, in the course of its preparation.

* This work was presented in part at the Illrd Congress of the International Society on Thrombosis and Haemostasis, Washington, U.S.A., August 1972.


** Present address; Inserm, Unité 63, 22 Avenue du Doyen Lépine, 69500 Lyon-Bron-France.


 

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