The Presence of Glycogen Synthase in Preparations of Platelet Plasma Membranes

J. H Greenberg; A. P Fletcher; G. A Jamieson

doi:10.1055/s-0038-1649079

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1973; 30(02): 307-314
DOI: 10.1055/s-0038-1649079

Original Article

Schattauer GmbH

The Presence of Glycogen Synthase in Preparations of Platelet Plasma Membranes

J. H Greenberg

¹American National Red Cross Blood Research Laboratory, 9312 Old Georgetown Road, Bethesda, Maryland 20014

,

A. P Fletcher

¹American National Red Cross Blood Research Laboratory, 9312 Old Georgetown Road, Bethesda, Maryland 20014

,

G. A Jamieson

¹American National Red Cross Blood Research Laboratory, 9312 Old Georgetown Road, Bethesda, Maryland 20014

› Author Affiliations

Abstract

Summary

Glycogen synthase and glycogen (63 ± 35 μg/unit) have been identified in partially-purified preparations of platelet membranes isolated by density-step centrifugation following glycerol-lysis; this combination is responsible for the endogenous (collagen-independent) activity measured during the assay of collagen: glucosyltransferase. Glycogen synthesized in this way may be complexed with protein since it is precip-itable with trichloracetic acid. High and low density membranes prepared by continuous density gradient centrifugation are devoid of glycogen synthase activity.

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References

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