Platelet Factor 3 Activity Made Available from Human Platelets by ADP

Summary

Pretreament of normal human platelets with colchicine at a final concentration of 10^-3 M strikingly modifies their subsequent reaction to ADP. The generation of platelet factor 3 activity is inhibited, the secondary wave of aggregation fails to appear, and platelet factor 4 is not released. Colchicine also inhibits the increase in platelet factor 3 activity that results from washing and centrifugation of platelets.

^* Present address : Albert Einstein School of Medicine, Bronx, New York.

^** Present address : Medical Department A, Rikshospitalet, Oslo, Norway.

• References

• 1 Behnke O. 1970; Effects of some chemicals on blood platelet microtubules, platelet shape and some platelet functions in vitro. Scandinavian Journal of Haematology 7: 123.
• 2 Booyse F. M, Hoveke T. P, Kisieleski D, and Rafelson Jr. M. E. 1972; Mechanism and control of platelet-platelet interaction. I. Effects of inducers and inhibitors of aggregation. Microvascular Research 4: 179.
• 3 Boeisy G. G, and Taylor E. W. 1967; The mechanism of action of colchicine. Binding of colchicine - ³H to cellular protein. Journal of Cell Biology 34: 525.
• 4 Gillespie E, Levine R. J, and Malawista S. E. 1968; Histamine release from rat peritoneal mast cells: inhibition by colchicine and potentiation by deuterium oxide. Journal of Pharmacology and Experimental Theraputics 177: 122.
• 5 Hovie T. 1969; Effects of in vivo administration of colchicine on the morphology and function of blood platelets. Abstracts. Journal of Ultrastructure Research 29: 574.
• 6 Lacy P. E, Howell S. L, Young D. A, and Fink C. J. 1968; New hypothesis of insulin secretion. Nature (London) 219: 1177.
• 7 Malawista S. E. 1965; On the action of colchicine. The melanocyte model. Journal of Experimental Medicine 122: 361.
• 8 Marcus A. J, Zucker-Franklin D, Safier L. B, and Ullman H. L. 1966; Studies on human platelet granules and membranes. Journal of Clinical Investigation 45: 14.
• 9 Niewiarowski S, and Thomas D. P. 1969; Platelet factor 4 and adenosine diphosphate release during human platelet aggregation. Nature (London) 222: 1269.
• 10 Poisner A. M, and Bernstein J. 1971; A possible role of microtubules in catecholamine release from the adrenal medulla: effect of colchicine, vinca alkaloids and deuterium oxide. Journal of Pharmacology and Experimental Theraputics 177: 102.
• 11 Shepro D, Belamarich F. A, and Chao F. C. 1969; Retardation of clot retraction after incubation of platelets with colchicine and heavy water. Nature (London) 221: 563.
• 12 Shepro D, Ruderman F. R, Kien M, and Belamarich F. A. 1971. Antimotility agents, platelet shape change and aggregation. In: Proceedings of the 6th European Conference on Microcirculation, Aalborg, 1970,. Karger; Basel: 314.
• 13 Sixma J. J, and Nijessen F. G. 1970; Characteristics of platelet factor 3 release during ADP-induced aggregation. Comparison with 5 hydroxytryptamine release. Thrombosis et Diathesis Haemorrhagica 24: 206.
• 14 Sneddon J. M. 1971; Effect of mitosis inhibitors on blood platelet microtubules and aggregation. Journal of Physiology 214: 145.
• 15 Soppitt G. D, and Mitchell J. R. A. 1969; The effect of colchicine on human platelet behavior. Journal of Atherosclerosis Research 10: 247.
• 16 Tilney L. G. 1968; Studies on the microtubules of heliozoa. IV. The effect of colchicine on the formation and maintenance of the axopodia. Journal of Cell Science 3: 549.
• 17 Walsh P. N. 1972; The effect of dilution of plasma on coagulation: the significance of the dilution-activation phenomenon for the study of platelet coagulant activities. British Journal of Haematology 22: 219.
• 18 White F. G. 1968; Effects of colchicine and vinca alkaloids on human platelets. I. Influence on platelet microtubules and contractile function. American Journal of Pathology 53: 281.
• 19 White F. G. 1969; Effects of colchicine and vinca alkaloids on human platelets. III. Influence on primary internal contraction and secondary aggregation. American Journal of Pathology 54: 467.
• 20 Zucker-Franklin D. 1969; Microfibrils of blood platelets: their relationship to microtubules and the contractile protein. Journal of Clinical Investigation 48: 165.