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Thromb Haemost 1973; 30(02): 414-424
DOI: 10.1055/s-0038-1649120
Original Article
Schattauer GmbH

Studies on an Inhibitor of Plasminogen Activation in Human Serum

Ulla Hedner
1   Coagulation Laboratory, Allmänna Sjukhuxet, Malmö, Sweden
› Institutsangaben
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Publikationsverlauf

Received for publication 22. März 1973

Accepted for publication 28. Juni 1973

Publikationsdatum:
30. Juni 2018 (online)

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Summary

A procedure is described for partial purification of an inhibitor of the activation of plasminogen by urokinase and streptokinase. The method involves specific adsorption of contammants, ion-exchange chromatography on DEAE-Sephadex, gel filtration on Sephadex G-200 and preparative electrophoresis. The inhibitor fraction contained no antiplasmin, no plasminogen, no α1-antitrypsin, no antithrombin-III and was shown not to be α2 M or inter-α-inhibitor. It contained traces of prothrombin and cerulo-plasmin. An antiserum against the inhibitor fraction capable of neutralising the inhibitor in serum was raised in rabbits.

 

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