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Thromb Haemost 1973; 30(03): 425-436
DOI: 10.1055/s-0038-1649121
Original Article
Schattauer GmbH

Human Prothrombin: A New Method of Preparation from a Single Individual

Richard Benarous
1   Institut de Pathologie Moléculaire* 24, rue du Faubourg Saint-Jacques, 75014 Paris, France
2   UER Necker, Dept of Hematology, 149, rue de Sèvres, 75007 Paris, France
,
Dominique Labie
1   Institut de Pathologie Moléculaire* 24, rue du Faubourg Saint-Jacques, 75014 Paris, France
2   UER Necker, Dept of Hematology, 149, rue de Sèvres, 75007 Paris, France
,
François Josso
1   Institut de Pathologie Moléculaire* 24, rue du Faubourg Saint-Jacques, 75014 Paris, France
2   UER Necker, Dept of Hematology, 149, rue de Sèvres, 75007 Paris, France
› Author Affiliations
Further Information

Publication History

Received 12 June 1973

Accepted 03 August 1973

Publication Date:
30 June 2018 (online)

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Summary

A simple method for purification of human prothrombin from ACT) plasma of one single individual is described. This method is based on three steps :

1. isolation of crude prothrombin complex by barium citrate adsorption;

2. first purification by hydroxyapatite chromatography;

3. further purification by DEAE Sephadex chromatography.

The final product was obtained with a good yield and exhibited less than 5 p. cent contamination. The human purified prothrombin obtained in this way displays :

– one single chain by SDS electrophoresis,

– a molecular weight of 75,000-80,000 daltons,

– a pi value pH 4.4 by electrofocusing,

– Alanine as थ terminal residue.

Amino-acid analysis is reported.

* Institut d’Université, Groupe U 15 de l’Institut National de la Santé et de la Recherche Médicale, Laboratoire associé au Centre National de la Recherche Scientifique.


 

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