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Thromb Haemost 1973; 30(03): 437-450
DOI: 10.1055/s-0038-1649122
Original Article
Schattauer GmbH

Human Coumarin Prothrombin

Chromatographic, Coagulation and Immunologic Studies
Nicole Cesbron
1   Service Central d’ Immunologie et Hématologie, Hôpital Beaujon, 92/Clichy and CHU Xavier Bichat, Paris, France
,
Catherine Boyer
1   Service Central d’ Immunologie et Hématologie, Hôpital Beaujon, 92/Clichy and CHU Xavier Bichat, Paris, France
,
Marie-Claude Guillin
1   Service Central d’ Immunologie et Hématologie, Hôpital Beaujon, 92/Clichy and CHU Xavier Bichat, Paris, France
,
Doris Ménaché
1   Service Central d’ Immunologie et Hématologie, Hôpital Beaujon, 92/Clichy and CHU Xavier Bichat, Paris, France
› Author Affiliations
Further Information

Publication History

Received 16 July 1973

Accepted 05 September 1973

Publication Date:
30 June 2018 (online)

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Summary

The abnormal prothrombin produced in patients under coumarin treatment has been isolated from oxalated plasma. After removal of normal prothrombin by adsorption on to small amounts of barium sulfate, coumarin prothrombin proved to be adsorbable on to larger amounts of barium sulfate. After elution into citrate the coumarin prothrombin was precipitated with ethanol and chromatographed on DEAE cellulose. Using a similar linear gradient than for normal prothrombin, the coumarin prothrombin was eluted ahead of normal prothrombin. Immunological studies performed on chromatographed coumarin prothrombin showed that it shared with normal prothrombin the main antigenic determinants and a complete line of identity was obtained when tested against antihuman normal prothrombin antiserum. On crossed Immunoelectrophoresis the electrophoretic mobility of coumarin prothrombin, unlike normal prothrombin, was not modified by the addition of calcium ions. Coagulation studies revealed that coumarin prothrombin could not be activated by physiological activators whereas conversion to thrombin was possible by non physiological activators such as staphylocoagulase and Echis carinatus venom. The amounts of thrombin generated under staphylocoagulase activation per 100 antigen units were similar to those generated by normal prothrombin. The amounts of thrombin obtained under the action of the venom were less than from normal prothrombin. In addition, the activation of coumarin prothrombin proved to be slower than for normal prothrombin.

 

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