Thromb Haemost 1973; 30(03): 471-479
DOI: 10.1055/s-0038-1649125
Original Article
Schattauer GmbH

The Measurement of Heparin

A Method Based on the Potentiation of Anti-Factor Xa
K. W. E Denson
1   John Radcliffe Hospital, Oxford
,
John Bonnar
1   John Radcliffe Hospital, Oxford
› Author Affiliations
Further Information

Publication History

Received 24 July 1973

Accepted 20 August 1973

Publication Date:
30 June 2018 (online)

Summary

A method for the measurement of heparin utilising the potentiating effect of heparin on the action of anti-factor Xa is described. The effect on the assay of platelet contamination of plasma, the presence of fibrinogen degradation products and low levels of anti-factor Xa have been studied. The assay method has been compared with the calcium thrombin time method and a group of obstetrical patients have been studied using both methods.

 
  • References

  • 1 Biggs R, Denson K. W. E, Ackman N, Borrett R, and Haddon M. 1970; Antithrombin III, Antifactor Xa and Heparin. British Journal of Haematology 19: 283.
  • 2 Eika C, Godal H. C, and Kierulf P. 1972; Detection of Small Amounts of Heparin by the Thrombin Clotting Time. Lancet II: 376.
  • 3 Gordon-Smith I. C, le Quesne L. P, Grundy D. J, Newcombe J. F, and Bramble F. J. 1972; Controlled Trial of Two Regimens of Subcutaneous Heparin in Prevention of Post-Operative Deep-Vein Thrombosis. Lancet I: 1133.
  • 4 Kakkar V. V, Nicholaides A. N, Field E. S, Flute P. T, Wessler S, and Yin E. T. 1971; Low Doses of Heparin in Prevention of Deep-Vein Thrombosis. Lancet II: 669.
  • 5 Kakkar V. V, Spindler J, Flute P. T, Corrigan T, Fossard D. P, Grellin R. Q, Wessler S, and Yin E. T. 1972; Efficiency of Low Doses of Heparin in Prevention of Deep-Vein Thrombosis after Major Surgery. Lancet II: 101.
  • 6 Walsh P. H. 1972; Albumin Density Gradient Separation and Washing of Platelets and the Study of Platelet Coagulant Activities. British Journal of Haematology 22: 205.
  • 7 Yin E. T, and Wessler S. 1970; Heparin accelerated inhibition of activated factor X by its natural plasma inhibitor. Biochimica et Biophysica Acta 201: 287.
  • 8 Yin E. T, Wessler S, Butler J. V, and Cole S. 1973; Plasma Heparin: A unique, practical, submicrogram-sensitive assay. Journal of Laboratory and Clinical Medicine 81: 298.