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DOI: 10.1055/s-0038-1649135
Platelets and Clot Retraction
Effect of Divalent Cations and Several DrugsPublikationsverlauf
Received
09. April 1973
Accepted
01. Juni 1973
Publikationsdatum:
30. Juni 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/197302/lookinside/thumbnails/10-1055-s-0038-1649135-1.jpg)
Summary
The effect of Ca2+, Mg2+, Mn2+ and some drugs known to influence smooth muscle behaviour on human and rat whole blood and PRP clot retraction was studied.
The force of the retraction was measured by a dynographic apparatus using electrical transduction. Clot retraction was studied by thrombelastographic method as well.
The results obtained by using EDTA and EGT A show that both Ca2+ and Mg2+ are necessary for the normal development of clot retraction. They suggest the coexistence in the platelets of an accessible calcium fraction and a fraction not readily accessible to chelating agents, which is released following the calcium chelation in the first fraction.
MnCl2 inhibits clot retraction and induced a quick, complete and irreversible relaxation of fully contracted clots. It is concluded that there are some analogies between blood clots and smooth muscle.
The absence of any effect of adrenaline, noradrenaline and ADP, which strongly modify smooth muscle contraction, is not surprising. This is probably because clotting caused by thrombin-triggered platelets has already produced a maximal clot retraction.
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References
- 1 Axelsson J, and Högberg S. G. R. 1967; Effects of Caffeine on Electrical and Mechanical Activity of Smooth Muscle. Acta Pharmacologica and Toxicológica 25 Suppl. (04) 53.
- 2 Biggs R, and MacFaelane R. G. 1962. Human Blood Coagulation and Its Disorders. 3rd edn.. Blackwell Scientific Publications; Oxford:
- 3 Bottecchia D, and Doni M. G. 1973 Artero-venous Differences in Blood Platelet Clumping by ADP. Experientia, accepted for publication.
- 4 Beading A, Bülbring E, and Tomita T. 1969; The Effect of Sodium and Calcium on the Action Potential of the Smooth Muscle of the Guinea-pig Taenia Coli. Journal of Physiology 200: 637.
- 5 Bülbring E, and Tomita T. 1968; The Effects of Ba2+ and Mn2+ on the Smooth Muscle of the Guinea-pig Taenia Coli. Journal of Physiology 196: 137.
- 6 Daniel E. E. 1964. Attempted Synthesis of Data Regarding Divalent Ions in Muscle Function. In: Muscle,. Symposium Publications Division Pergamon Press; Oxford, London, New York:
- 7 Davidsohn I, and Nelson D. A. 1969. The Blood. In: Clinical Diagnosis by Laboratory Methods. 14th edn.. W. B. Saunders Company; Philadelphia, London, Toronto:
- 8 de Gaetano G, Bottecchia D, and Vermylen J. 1973 a Retraction of Reptilase-clots in the Presence of Agents Inducing or Inhibiting the Platelet Adhesion-aggregation Reaction. Thrombosis Research, accepted for publication.
- 9 de Gaetano G, Donati M. B, Bottecchia D, and Vermylen J. 1973 b New Methods for Evaluating the Platelet Adhesion-aggregation Reaction. Symposium on Platelet Aggregation and Drugs, Roma 7—8 march 1973.
- 10 Grette K. 1962; Studies on the Mechanism of Thrombin-catalyzed Hemostatic Reactions in Blood Platelets. Acta Physiologica Scandinavica 56 (Suppl.) 195.
- 11 Imai S, and Takeda K. 1967; Effects of Vasodilatators on the Isolated Taenia Coli of the Guinea-pig. Nature, London 213: 509.
- 12 Kuriyama K. 1970. Effects of Ions and Drugs on the Electrical Activity of Smooth Muscle. In: Smooth Muscle,. Edward Arnold; London:
- 13 Lüscher E. F. 1971. Biochemical Basis of Platelet Function. In: The Platelet. The Williams & Wilkins Company; Baltimore:
- 14 Lüscher E. F, and Bettex-Galland M. 1971. Thrombosthenin. In: The Circulating Platelet. Academic Press; New York and London:
- 15 Lüscher E. F, Probst E, and Bettex-Galland M. 1972; Thrombosthenin: Structure and Function. Annals of the New York Academy of Sciences 201: 122.
- 16 Majno G, Bouvier C. A, Gabbiani G, Ryan G. B, and Statkov P. 1972; Kymographic Recording of Clot Retraction: Effects of Papaverine, Theophylline and Cytochalasin B. Thrombosis and Diathesis Haemorragica 28: 49.
- 17 Mustard J. F, and Packam M. A. 1970; Factors Influencing Platelet Function: Adhesion, Release, and Aggregation. Pharmacological Reviews 22: 97.
- 18 Odell Jr. T. T, and Upton A. C. 1955; Distribution of Calcium45 in Platelets and Bone Marrow of Rats. Acta Haematologica 14: 291.
- 19 Somlyo A. P, and Somlyo A. V. 1970; Vascular Smooth Muscle. II. Pharmacology of Normal and Hypertensive Vessels. Pharmacological Reviews 22: 249.
- 20 Wallach D. F. H, Surgenor D. M, and Steele B. B. 1958; Calcium-lipid Complexes in Human Platelets. Blood 13: 589.
- 21 Wintrobe M. M. 1967. Clinical Hematology. 6th edn.. Lea and Febiger; Philadelphia: