Summary
When rabbit platelet-rich plasma was treated with 1-2 mM molecular iodine, marked irreversible aggregation of platelets was observed. The aggregation was characterized by inhibition by 1 mM reduced glutathione, acceleration by 0.1 mM NEM, and no interference with EDTA and adenosine which are powerful inhibitors of aggregation induced by ADP. Molecular iodine at a concentration of 0.5 mM did not induce aggregation and caused more than 60% inhibition of ADP- and collagen-induced aggregation. These findings indicated that the aggregation by molecular iodine was in quite different mechanisms from those by other aggregating agents such as ADP, and suggested that platelet aggregation by molecular iodine and ADP was greatly regulated by sulfhydryl groups.
