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Thromb Haemost 1974; 31(03): 493-504
DOI: 10.1055/s-0038-1649189
Original Article
Schattauer GmbH

Platelet Adhesion from Nonanticoagulated Rat Arterial Blood*

Murray M. Bern
1   Department of Medicine New England Deaconess Hospital, Boston, Massachusetts
2   The Center for Blood Research, 800 Huntington Avenue, Boston, Massachusetts
,
James L. Tullis
1   Department of Medicine New England Deaconess Hospital, Boston, Massachusetts
2   The Center for Blood Research, 800 Huntington Avenue, Boston, Massachusetts
› Author Affiliations
Further Information

Publication History

Received 04 February 1974

Accepted 10 March 1974

Publication Date:
29 June 2018 (online)

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Summary

Platelet adhesion to glass bead is measured using nonanticoagulated arterial blood from adult male rats. Parameters studied include: 1) surface area of glass exposed to platelets, 2) time of contact and rate of platelet flow over the beads, 3) differing blood-gas interface, 4) adhesion to siliconized polyvinyl surfaces, and 5) heparinization of donors. Parameters kept constant include: 1) diameter of glass beads, 2) diameter of glass bead chamber, and 3) body temperature of the donor animals. No air was mixed with the blood. Concentration of hemoglobin released into the plasma supernatant was measured.

Platelet adhesion from each aliquot of each sample increases as glass surface area increases, and the time of contact between the platelets and surface increases. Adhesion did not change when nitrogen was substituted for room air at blood-gas interface. Heparinization of donor animal did not alter adhesion. Platelets adhere to siliconized polyvinyl at greater rate than to nonsiliconized polyvinyl. Hemoglobin concentration in the plasma supernate did not correlate directly with percent of platelet adhesion. These results are similar to those reported for native and anti-coagulated venous and anticoagulated arterial blood.

This technique does not require in vitro handling of platelets. It can be applied to other animals and to humans.

* * Send requests for reprints to Murray M. Bern, M. D., New England Deaconess Hospital, Department of Medicine, 185 Pilgrim Road, Boston, Massachusetts, 02215.


 

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