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DOI: 10.1055/s-0038-1649210
The Reversible Antithrombin Activity of Incubated Plasma
Publikationsverlauf
Received 12. Januar 1976
Accepted 03. September 1976
Publikationsdatum:
03. Juli 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/197701/lookinside/thumbnails/10-1055-s-0038-1649210-1.jpg)
Summary
The thrombin time of normal citrated plasma is progressively prolonged on incubation in open glass tubes at 37° C. The phenomenon is dependent on the temperature and duration of incubation, on the pH, and on the concentration of calcium ions present. Platelet-rich citrated plasma fails to exhibit augmented antithrombin activity when similarly incubated, and the addition of washed platelets to platelet-poor plasma inhibits this activity. The clot retarding action of incubated plasma against thrombin is also manifested against Arvin (Ancrod), but not against Reptilase-R. This thrombin time lenghening may be inhibited by incubation with anti-antithrombin III antiserum thus indicating that the phenomenon of thrombin time lengthening is consistent with enhanced activity of antithrombin III. It is unlikely that alterations in the activity of α2-macroglobulin, is important in the reduced thrombin-coagulability of incubated plasma. Interference with the polymerisation of fibrin monomers by the physicochemical changes may contribute to the observed phenomenon.
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