Evaluation of a New Anti-Fibrinogen-Coated Latex Particle Agglutination Test in the Measurement of Serum Fibrin Degradation Products

Victor J. Marder; Genuina O. Cruz; Bruce R. Schumer

doi:10.1055/s-0038-1649218

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1977; 37(02): 183-191
DOI: 10.1055/s-0038-1649218

Original Article

Schattauer GmbH

Evaluation of a New Anti-Fibrinogen-Coated Latex Particle Agglutination Test in the Measurement of Serum Fibrin Degradation Products

Autoren

Victor J. Marder

¹Department of Medicine and the Specialized Center of Research in Thrombosis, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140 U.S.A.
Genuina O. Cruz

¹Department of Medicine and the Specialized Center of Research in Thrombosis, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140 U.S.A.
Bruce R. Schumer

¹Department of Medicine and the Specialized Center of Research in Thrombosis, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140 U.S.A.

Abstract

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Summary

A new latex particle agglutination test (FIBROTEX®) has been compared with the Thrombo-Wellcotest® and the tanned red cell hemagglutination inhibition immunoassay (TRCHII) for sensitivity to purified fibrinogen and plasmic degradation products and for reactivity with 31 normal sera and 170 sera from patients with thrombotic disorders. The latex particles in the FIBROTEX® are coated with antibodies against fibrinogen and fragment E (anti-FE), in contrast with the anti-fragment D and anti-fragment E antibodies which coat the latex particles of the Thrombo-Wellcotest® (anti-DE). The relative sensitivities of the TRCHII and the anti-FE and anti-DE latex tests were, respectively, 0.5, 2.5 and 7.5 μg/ml for fibrinogen; 0.5, 0.5 and 4.0 μg/ml for fragment X; and 1.25, > 30 and 8.0 μg/ml for fragment D. Compared with anti-DE, the anti-FE particles demonstrated fewer false-positive results with sera and urines of normals and higher titers, fewer false negatives and better overall correlation with the TRCHII results (r = 0.89 vs. 0.75) on pathologic sera. The FIBROTEX® is simple and rapid (5 minutes) and provides a more sensitive and accurate determination of serum fibrin degradation products than the Thrombo-Wellcotest®.

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Referenzen

References
1 Arocha-Pinango C.L. A comparison of the TRCHII and latexparticle tests for the titration of FR-antigen. Journal of Clinical Pathology 1972; 25: 757
2 Castelan D.J, Hirsh J, Martin M. Latex-bound antifibrinogen test for plasma fibrinogen assay. Journal of Clinical Pathology 1968; 21: 638
3 Clarkson A.R, MacDonald M.K, PEtrie J.J.B, Cash J.D, Robson J.S. Serum and urinary fibrin/fibrinogen degradation products in glomerulonephritis. British Medical Journal 1971; 3: 447
4 Donati M.B, Semeraro N, Vermylen J. Detection of fibrinogen antigens with two latex techniques applied to urine concentrates. Journal of Clinical Pathology 1973; 26: 760
5 Ellman L, Carvalho A, Colman R.W. The Thrombo-Wellcotest as a screening test for disseminated intravascular coagulation. The New England Journal of Medicine 1973; 288: 633
6 Erickson C, String T, Stewart D, Cohen R.J. Evaluation of methods for the detection and quantitation of serum fibrin-fibrinogen degradation products. American Journal of Clinical Pathology 1972; 58: 394
7 Garvey M.B, Black J.M. The detection of fibrinogen/fibrin degradation products by means of a new antibody-coated latex particle. Journal of Clinical Pathology 1972; 25: 680
8 Hoq M.S, Cash J.D. Studies on a direct latex agglutination technique for the semiquantita-tion of fibrin/fibrinogen degradation products. Thrombosis Research 1973; 2: 23
9 Hulme B, Pitcher P.M. Rapid latex-screening test for detection of fibrin/fibrinogen degradation products in urine after renal transplantation. Lancet 1973; I: 6
10 Johnson A.J, Kline D.L, Alkjaersig N. Assay methods and standard preparations for plasmin, plasminogen and urokinase in purified systems, 1967–1968. Thrombosis et Diathesis Haemorrhagica 1969; 21: 259
11 Marder V.J, James H.L, Sherry S. The purification of fibrinogen degradation products by Pevikin block electrophoresis. Thrombosis et Diathesis Haemorrhagica 1969; 22: 234
12 Marder V.J, Shulman N.R, Carroll W.R. High molecular weight derivatives of human fibrinogen produced by plasmin. I. Physicochemical and immunological characterization. Journal of Biological Chemistry 1969; 244: 2111
13 Marder V.J, Matchett M.O, Sherry S. Detection of serum fibrinogen and fibrin degradation products. Comparison of six technics using purified products and application in clinical studies. The American Journal of Medicine 1971; 51: 71
14 Merskey C, Kleiner G.J, Johnson A.J. Quantitative estimation of split products of fibrinogen in human serum, relation to diagnosis and treatment. Blood 1966; 28: 1
15 Merskey C, Lalezari P, Johnson A.J. A rapid, simple, sensitive method for measuring fibrinolytic split products in human serum. Proceedings of the Society of Experimental Biology and Medicine 1969; 131: 871
16 Pitcher P.M. The detection of fibrinogen degradation products (FDP) in serum and urine. Canadian Journal of Medical Technology 1972; 34: 166
17 Smith R.T, Ts’ao C.-H. Fibrin degradation products in the postoperative period. Evaluation of a new latex agglutination method. American Journal of Clinical Pathology 1973; 60: 644
18 Thomas D.P, Niewiarowski S, Myers A.R, Bloch K.J, Colman R.W. A comparative study of four methods for detecting fibrinogen degradation products in patients with various diseases. The New England Journal of Medicine 1970; 283: 663