Evaluation of a New Anti-Fibrinogen-Coated Latex Particle Agglutination Test in the Measurement of Serum Fibrin Degradation Products

Victor J. Marder; Genuina O. Cruz; Bruce R. Schumer

doi:10.1055/s-0038-1649218

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1977; 37(02): 183-191
DOI: 10.1055/s-0038-1649218

Original Article

Schattauer GmbH

Evaluation of a New Anti-Fibrinogen-Coated Latex Particle Agglutination Test in the Measurement of Serum Fibrin Degradation Products

Victor J. Marder

¹Department of Medicine and the Specialized Center of Research in Thrombosis, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140 U.S.A.

,

Genuina O. Cruz

¹Department of Medicine and the Specialized Center of Research in Thrombosis, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140 U.S.A.

,

Bruce R. Schumer

¹Department of Medicine and the Specialized Center of Research in Thrombosis, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140 U.S.A.

› Author Affiliations

Abstract

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Summary

A new latex particle agglutination test (FIBROTEX®) has been compared with the Thrombo-Wellcotest® and the tanned red cell hemagglutination inhibition immunoassay (TRCHII) for sensitivity to purified fibrinogen and plasmic degradation products and for reactivity with 31 normal sera and 170 sera from patients with thrombotic disorders. The latex particles in the FIBROTEX® are coated with antibodies against fibrinogen and fragment E (anti-FE), in contrast with the anti-fragment D and anti-fragment E antibodies which coat the latex particles of the Thrombo-Wellcotest® (anti-DE). The relative sensitivities of the TRCHII and the anti-FE and anti-DE latex tests were, respectively, 0.5, 2.5 and 7.5 μg/ml for fibrinogen; 0.5, 0.5 and 4.0 μg/ml for fragment X; and 1.25, > 30 and 8.0 μg/ml for fragment D. Compared with anti-DE, the anti-FE particles demonstrated fewer false-positive results with sera and urines of normals and higher titers, fewer false negatives and better overall correlation with the TRCHII results (r = 0.89 vs. 0.75) on pathologic sera. The FIBROTEX® is simple and rapid (5 minutes) and provides a more sensitive and accurate determination of serum fibrin degradation products than the Thrombo-Wellcotest®.

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References

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