Binding of Anti-Actin Autoantibodies to Platelets

Claude A Bouvier; Giulio Gabbiani; Graeme B Ryan; Marie-Claude Badonnel; Guido Majno; Ernst F Lüscher

doi:10.1055/s-0038-1649232

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1977; 37(02): 321-328
DOI: 10.1055/s-0038-1649232

Original Article

Schattauer GmbH

Binding of Anti-Actin Autoantibodies to Platelets

Claude A Bouvier

¹Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland

,

Giulio Gabbiani

¹Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland

,

Graeme B Ryan

¹Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland

,

Marie-Claude Badonnel

¹Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland

,

Guido Majno

¹Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland

,

Ernst F Lüscher

¹Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland

› Author Affiliations

Abstract

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Summary

Normal platelets incubated with anti-actin autoantibodies (AAA) (from the serum of patients with chronic aggressive hepatitis) do not show binding of these antibodies as seen by indirect immunofluorescence. AAA serum does not inhibit thrombin-induced clot retraction, despite the binding of the antibodies to platelets in the clot. Similarly, AAA serum does not affect “reversible” or “irreversible” aggregation (induced by ADP, collagen or epinephrine), despite the binding of the antibodies to platelet actin under such circumstances. AAA also bind to platelets when aggregation is inhibited by EDTA. The incubation of “reversibly” aggregated platelet with AAA results in a small but definite binding of AAA to platelets. These findings suggest that during “irreversible” and/or “reversible” aggregation, changes take place at the surface of platelets which expose the antigen at the surface of the cell.

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References

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