Cyanide Insensitive Oxidase in Platelets of Newborn Infant

D Del Principe; A Menichelli; A. M Damiano; L Coppola; G Sabetta

doi:10.1055/s-0038-1649234

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1977; 37(02): 339-343
DOI: 10.1055/s-0038-1649234

Original Article

Schattauer GmbH

Cyanide Insensitive Oxidase in Platelets of Newborn Infant

D Del Principe

¹Institute of Pediatrics, Medical School, University of Rome, Italy.

,

A Menichelli

¹Institute of Pediatrics, Medical School, University of Rome, Italy.

,

A. M Damiano

¹Institute of Pediatrics, Medical School, University of Rome, Italy.

,

L Coppola

¹Institute of Pediatrics, Medical School, University of Rome, Italy.

,

G Sabetta

¹Institute of Pediatrics, Medical School, University of Rome, Italy.

› Author Affiliations

Abstract

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Summary

The authors studied the behaviour of neonate platelet O₂ consumption after the addition of pyridine nucleotide compared to adult controls.

O₂ consumption of neonate platelets after NADH addition was 103,2 mμmol O₂/10⁹/hr (SE = 24,74) and in adult controls 188,8 mμmol O₂/10⁹/hr (SE = 36,46).

After the addition of NADPH O₂ consumption was, respectively, 233,5 mμmol (SE = 46,29) and 218,3 mμmol (SE = 30,01).

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References

References
1 Baehner R. L, Gilman N, Karnowski M. L. Respiration and glucose oxidation in human and guinea pig leukocytes: comparative studies. Journal Clinical Investigation 1970; 49: 692
2 Crowley J. P, Skrabut E. M, Valeri C. R. The determination of leukocyte phagocytic oxidase activity by measurement of the initial rate of stimulated oxygen consumption. Journal Laboratory Clinical Medicine 1975; 86: 586
3 Dejesus M, Fikrig S, Detwiller T. Phagocytosis stimulated nitroblue tetrazolium reduction by platelets. Journal Laboratory Clinical Medicine 1972; 80: 117
4 Del Principe D, Balducci L, Sabetta G. NBT test in newborn platelets. Thrombosis et Diathesis Haemorrhagica 1974; 31: 368
5 Hilgartner M. W. Transient functional thromboasthenia in the newborn. Society for Pediatric Research th Annual Meeting 1968; 38: 120
6 Hrodek O. Blood platelets in the newborn: their function in haemostasis and hemocoagulation. Acta Universitatis Carolinae Monograph 1966: 22
7 Humbert J. R, Gross G. P, Vatter A. E, Hathaway W. E. Nitroblue tetrazolium reduction by neutrophils: Biochemical and ultrastructural effects of methylene blue. Journal Laboratory Clinical Medicine 1973; 82: 20
8 Kitchens C. S, Newcomb T. F. Human platelet respiration. Journal Applied Physiology 1968; 25: 581
9 Kuramoto A, Steiner M, Baldini M. G. Lack of platelet response to stimulation in the Wiskott-Aldrich syndrome. New England Journal Medicine 1970; 282: 475
10 Mull N. M, Hathaway W. E. Altered platelet function in newborn. Pediatric Research 1970; 4: 229
11 Murano G, Berni-Canani M. Peculiarità funzionali e metaboliche delle piastrine del neonato. La Pediatría 1966; 74: 353
12 Oski F. A, Murphy S, Gardner F. H. Enzyme activity in the platelets of newborn infants. Pediatrics 1970; 45: 412
13 Santangelo G, Barberi I. Patología piastrinica nell’ età neonatale. Atti XXXV Congresso della Società Italiana di Pediatría 1972: 98
14 Sbarra A. J, Karnovski M. L. The biochemical basis of phagocytosis I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. Journal Biological Chemistry 1959; 234: 1355
15 Ts’Ao C, Green D, Schultz K. Function and ultrastructure of platelets of neonate: enhanced ristocetin aggregation of neonate platelets. British Journal of Haematology 1976; 32: 225
16 Whaun J. M. The platelet of the newborn infant 5-Hydroxytryptamine uptake and release. Thrombosis et Diathesis Haemorrhagica 1973; 30: 327
17 Zatti M, Rossi F, Meneghelli V. Metabolic and morphological changes of polymorphonuclear leukocytes during phagocytosis. British Journal Experimental Pathology 1965; 46: 227
18 Zatti M, Rossi F. Mechanism of the respiratory stimulation in phagocytosing leukocytes. The KCN-insensitive oxidation of NADPH₂ . Experientia 1966; 22: 758