In Vitro and in Vivo Induction of Cryofibrinogen and “Paracoagulation” by Reptilase

A.J Harder; P.W Straub

doi:10.1055/s-0038-1649372

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1972; 27(02): 337-348
DOI: 10.1055/s-0038-1649372

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

In Vitro and in Vivo Induction of Cryofibrinogen and “Paracoagulation” by Reptilase^[*]

A.J Harder

¹Department of Medicine, Kantonsspital, University of Zurich, Switzerland

,

P.W Straub

¹Department of Medicine, Kantonsspital, University of Zurich, Switzerland

› Author Affiliations

Abstract

Summary

Small doses of Reptilase, a snake venom enzyme which exclusively liberates fibrinopeptide A from fibrinogen, leads to formation of cryofibrinogen as well as ethanol- and protamine-precipitability of plasma both in vitro and in vivo. Precipitates obtained in vitro were shown to contain both fibrinogen and fibrin monomer. At 20° C Reptilase-treated fibrinogen solutions contained complexes with a sedimentation rate of 11-11.6 s_20W, similar to those observed after thrombin. The artificial production of cryofibrinogenemia in volunteers is discussed in relation to spontaneously occurring cryofibrinogenemias in patients with intravascular coagulation.

Full Text

References

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In Vitro and in Vivo Induction of Cryofibrinogen and “Paracoagulation” by Reptilase[*]

In Vitro and in Vivo Induction of Cryofibrinogen and “Paracoagulation” by Reptilase^[*]