Some Relations Between Light Transmission Changes and Biochemical Parameters of Stored Platelet Packs

Summary

In the present work a computerised method for continuous monitoring of light transmission through platelet packs was evaluated. One series (Series 1) of 50 platelet concentrates was studied over a 5-day period, and thereafter the pH, extracellular lactate dehydrogenase (LDH) and platelet factor 4 (PF4) in the concentrates were determined. A second series (Series 2) of 52 concentrates was also monitored over 5 days, and thereafter the pH, extracellular LDH and intracellular concentrations of ATP, ADP and AMP were determined. 11 and 14 of the concentrates of Series 1 and 2, respectively, demonstrated long lasting transmission increases (≥ 3.0 days). In the total of 102 platelet concentrates 34 were observed to have late (<1.0 day) or no transmission increase. It is concluded, that the present optical method identifies both a group of platelet concentrates having long lasting transmission increases and a group consisting of platelet concentrates having late or no transmission increase. Compared to the latter concentrates preparations with long lasting transmission changes demonstrated significant biochemical alterations. These observations could be applicable in the quality control of platelet concentrates.

• References

• 1 Murphy S, Gardner FH. Platelet storage at 22°C: Role of gas transport across plastic Containers in maintenance of viability. Blood 1975; 46: 209-218
• 2 Grode G, Miripol J, Garber J, Barber T, Buchholz DH. Extended storage of platelets in a new plastic Container. I. Biochemical and morphologic changes. Transfusion 1985; 25: 204-208
• 3 Murphy S, Kahn RA, Holme S, Philips GL, Sherwood W, Davisson W, Buchholz DH. Improved storage of platelets for transfusion in a new Container. Blood 1982; 60: 194-200
• 4 Snyder EL, Ezekowitz M, Aster R, Murphy S, Ferri P, Smith E, Rzad L, Davisson W, Pope C, Kakaiya R, Buchholz DH. Extended storage of platelets in a new plastic Container. II. in vivo response to infusion of platelets stored for 5 days. Transfusion 1985; 25: 209-214
• 5 Fratantoni JC, Sturdivant B, Poindexter BJ. Aberrant morphology of platelets stored in five days containers. Thromb Res 1984; 33: 607-615
• 6 Frojmovic MM, Panjwani R. Blood cell structure-function studies: light transmission and attenuation coefficients of suspensions of blood cells and model particles at rest and with stirring. J Lab Clin Med 1975; 86: 326-347
• 7 Holme S, Murphy S. Quantitative measurements of platelet shape by light transmission studies; application to storage of platelets for transfusion. J Lab Clin Med 1978; 92: 53-64
• 8 Feinberg H, Sarin MM, Batka EA, Porter CR, Miripol JE, Stewart M. Platelet storage: Changes in cytosolic Ca²⁺ actin polymerization and shape. Blood 1988; 72: 766-769
• 9 Kunicki TJ, Tuccellini M, Becker GA, Aster RH. A study of variables affecting the quality of platelets stored at “room temperature”. Transfusion 1975; 15: 414-421
• 10 Fratantoni JC, Poindexter BJ, Bonner RF. Quantitative assessment of platelet morphology by light scattering: A potential method for the evaluation of platelets for transfusion. J Lab Clin Med 1984; 103: 620-631
• 11 Bellhouse EI, Inskip MJ, Davis JG, Entwistle CC. Pretransfusion non-invasive quality assessment of stored platelet concentrates. Br J Haematol 1987; 66: 503-508
• 12 Söhngen D, Lies M, Kretschmer V, Egbring R. Thrombozytenfunktion in vitro bei 5-Tage-Lagerung. Beitr Infusionsther 1987; 18: 163-166
• 13 Georg V, Holme S, Moroff G. Evaluation of two instruments for noninvasive platelet concentrate quality assessment. Transfusion 1989; 29: 273-275
• 14 Järemo P. Computerised method for monitoring stored platelet packs. Med Biol Eng Comput 1988; 26: 193-198
• 15 Solberg C, Østerud B, Little C. Platelet storage lesion: Formation of platelet fragments with platelet factor 3 activity. Thromb Res 1987; 48: 559-565
• 16 Shimizu T, Ishikawa Y, Morishima Y, Fukuda T, Kato K. Platelet factor 4 release from the platelets stored in platelet concentrates. Transfusion 1985; 25: 420-423
• 17 Holme S, Heaton WA, Courtright M. Platelet storage lesion in second generation containers: correlation with platelet ATP levels. Vox Sang 1988; 53: 214-220
• 18 The Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology. Scand J Clin Lab Invest 1974; 33: 291-306
• 19 Lundin A, Hasenson M, Persson J, Pousette Å. Estimation of biomass in growing cell lines by adenosine triphosphate assay. Methods Enzymol 1986; 133: 27-42
• 20 Holmsen H. Nucleotide metabolism of platelets. Annu Rev Physiol 1985; 47: 677-690
• 21 Rao AK, Niewiarowski S, Murphy S. Acquired granular pool defect in stored platelets. Blood 1981; 57: 203-208
• 22 Rucinski B, Niewiarowski S, James P, Walz DA, Budzynski AZ. Antiheparin proteins secreted by human platelets. Purification, characterization and radioimmunoassay. Blood 1979; 53: 47-62