Summary
In the present work a computerised method for continuous monitoring of light transmission
through platelet packs was evaluated. One series (Series 1) of 50 platelet concentrates
was studied over a 5-day period, and thereafter the pH, extracellular lactate dehydrogenase
(LDH) and platelet factor 4 (PF4) in the concentrates were determined. A second series
(Series 2) of 52 concentrates was also monitored over 5 days, and thereafter the pH,
extracellular LDH and intracellular concentrations of ATP, ADP and AMP were determined.
11 and 14 of the concentrates of Series 1 and 2, respectively, demonstrated long lasting
transmission increases (≥ 3.0 days). In the total of 102 platelet concentrates 34
were observed to have late (<1.0 day) or no transmission increase. It is concluded,
that the present optical method identifies both a group of platelet concentrates having
long lasting transmission increases and a group consisting of platelet concentrates
having late or no transmission increase. Compared to the latter concentrates preparations
with long lasting transmission changes demonstrated significant biochemical alterations.
These observations could be applicable in the quality control of platelet concentrates.
