Download PDF
Thromb Haemost 1993; 70(04): 676-680
DOI: 10.1055/s-0038-1649648
Original Article
Platelets
Schattauer GmbH Stuttgart

Influence of Platelet Membrane Sialic Acid and Platelet-Associated IgG on Ageing and Sequestration of Blood Platelets in Baboons

H F Kotzé
The Department of Haematology, Faculty of Medicine, University of the Orange Free State, Bloemfontein, Republic of South Africa
,
V van Wyk
The Department of Haematology, Faculty of Medicine, University of the Orange Free State, Bloemfontein, Republic of South Africa
,
P N Badenhorst
The Department of Haematology, Faculty of Medicine, University of the Orange Free State, Bloemfontein, Republic of South Africa
,
A du P Heyns
The Department of Haematology, Faculty of Medicine, University of the Orange Free State, Bloemfontein, Republic of South Africa
,
J P Roodt
The Department of Haematology, Faculty of Medicine, University of the Orange Free State, Bloemfontein, Republic of South Africa
,
M G Lötter
The Department of Haematology, Faculty of Medicine, University of the Orange Free State, Bloemfontein, Republic of South Africa
› Author Affiliations
Further Information

Publication History

Received 26 August 1992

Accepted after revision 01 June 1993

Publication Date:
05 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

Platelets were isolated from blood of baboons and treated with neuraminidase to remove platelet membrane sialic acid, a process which artificially ages the platelets. The platelets were then labelled with 111In and their mean life span, in vivo distribution and sites of Sequestration were measured. The effect of removal of sialic acid on the attachment of immunoglobulin to platelets were investigated and related to the Sequestration of the platelets by the spleen, liver, and bone marrow. Removal of sialic acid by neuraminidase did not affect the aggregation of platelets by agonists in vitro, nor their sites of Sequestration. The removal of 0.51 (median, range 0.01 to 2.10) nmol sialic acid/108 platelets shortened their life span by 75 h (median, range 0 to 132) h (n = 19, p <0.001), and there was an exponential correlation between the shortening of the mean platelet life span and the amount of sialic acid removed. The increase in platelet-associated IgG was 0.112 (median, range 0.007 to 0.309) fg/platelet (n = 25, p <0.001) after 0.79 (median, range 0.00 to 6.70) nmol sialic acid/108 platelets was removed (p <0.001). There was an exponential correlation between the shortening of mean platelet life span after the removal of sialic acid and the increase in platelet-associated IgG. The results suggest that platelet membrane sialic acid influences ageing of circulating platelets, and that the loss of sialic acid may have exposed a senescent cell antigen that binds IgG on the platelet membrane. The antibody-antigen complex may then provide a signal to the macrophages that the platelet is old, and can be phagocytosed and destroyed.

 

  • References

  • 1 Kay MMB. Isolation of the phagocytosis-inducing IgG-binding site in senescent somatic cells. Nature 1981; 289: 491-494
    CrossrefPubMedSearch in Google Scholar
  • 2 Aminoff D. The role of sialoglycoconjugates in the ageing and Sequestration of red cells from circulation. Blood Cells 1988; 14: 229-247
    PubMedSearch in Google Scholar
  • 3 Steiner M, Vancura S. Asymmetrical loss of sialic acid from membrane glycoproteins during platelet ageing. Thromb Res 1985; 40: 465-471
    CrossrefPubMedSearch in Google Scholar
  • 4 Reimers H-J, Greenberg JP, Cazenave J-P, Packham MA, Kinlough-Rathbone RL, Mustard JF. Experimental modification of platelet survival. Adv Exp Med Biol 1977; 82: 231-233
    PubMedSearch in Google Scholar
  • 5 Scott S, Reimers H-J, Chernesky MA, Greenberg JP, Kinlough-Rathbone RL, Packham MA, Mustard JF. Effect of viruses on platelet aggregation and platelet survival in rabbits. Blood 1978; 52: 47-55
    PubMedSearch in Google Scholar
  • 6 Greenberg JP, Packham MA, Guccione MA, Rand ML, Reimers H-J, Mustard JF. Survival of rabbit platelets treated in vitro with Chymotrypsin, plasmin, trypsin or neuraminidase. Blood 1979; 23: 916-927
    PubMedSearch in Google Scholar
  • 7 Rand ML, Greenberg JP, Packham MA, Mustard JP. Density subpopulations of rabbit platelets: Size, protein, and sialic acid content, and specific radioactivity changes following labelling with S-25-sulphate in vivo. Blood 1981; 57: 741-746
    PubMedSearch in Google Scholar
  • 8 Greenberg JP, Packham MA, Cazaenave J-P, Reimers H-J, Mustard JF. Effects on platelet function of removal of platelet sialic acid by neuraminidase. Lab Invest 1975; 32: 476-484
    PubMedSearch in Google Scholar
  • 9 Packham MA, Guccione MA, Kinlough-Rathbone RL, Mustard JF. Platelet sialic acid and platelet survival after aggregation by ADP. Blood 1980; 56: 876-880
    PubMedSearch in Google Scholar
  • 10 Nurden AT, George JN, Phillips DR. Platelet membrane glycoproteins: their structure, function and modification in disease. In: Biochemistry of Platelets. Phillips DR, Shuman MA. (eds) London: Academic Press Inc; 1986: 160-224
  • 11 Heyns A, Badenhorst PN, Wessels P, Pieters H, Lötter MG. Kinetics, in vivo distribution and sites of Sequestration of 111Indium-labelled platelets in giant platelet syndromes. Br J Haematol 1985; 60: 323-330
    CrossrefPubMedSearch in Google Scholar
  • 12 Kotzé HF, Lötter MG, Badenhorst PN, Heyns A duP. Kinetics of 111In-platelets in the baboon: I. Isolation and labelling of a viable and representative platelet population. Thromb Haemostas 1985; 53: 404-407
    PubMedSearch in Google Scholar
  • 13 Kotzé HF, Lötter MG, Badenhorst PN, Heyns A duP. Kinetics of 111In-platelets in the baboon: II. In vivo distribution and sites of Sequestration. Thromb Haemostas 1985; 53: 408-410
    PubMedSearch in Google Scholar
  • 14 Kotzé HF, Heyns A duP, Lötter MG, Pieters H, Roodt JP, Sweetlove MA, Badenhorst PN. Comparison of oxine and tropolone methods for labelling platelets with 111Indium. J Nucl Med 1991; 32: 62-66
    PubMedSearch in Google Scholar
  • 15 Warren L. Thiobarbituric acid assay of sialic acids. Methods Haematol 1963; VI: 463-465
    PubMedSearch in Google Scholar
  • 16 Lötter MG, Heyns A duP, Badenhorst PN, Wessels P, Van Zyl M, Kotzé HF, Minnaar PC. Evaluation of mathematical models to assess platelet function. J Nucl Med 1986; 27: 1192-1201
    PubMedSearch in Google Scholar
  • 17 Badenhorst PN, Pieters H. Methodology of platelet imaging. In: Platelet Kinetics and Imaging, Vol 1: Techniques and Normal Platelet Kinetics. Heyns A duP, Badenhorst PN, Lötter MG. (eds) Boca Raton, FL: CRC Press Inc; 1985: 159-168
  • 18 Nel JD, Stevens K. A new method for the simultaneous quantitation of platelet-bound immunoglobulin (IgG) and complement (C3) employing an enzyme-linked immunoabsorbent assay (ELISA) procedure. Br J Haematol 1980; 44: 281-290
    CrossrefPubMedSearch in Google Scholar
  • 19 Hanson SR, Kotzé HF, Savage B, Harker LA. Platelet interactions with Dacron vascular grafts. A model of acute thrombosis in babonns. Arteriosclerosis 1985; 5: 595-603
    CrossrefPubMedSearch in Google Scholar
  • 20 Nordt FJ, Franco M, Corfield A, Schauer R, Ruhenstroth-Bauer G. The efficacy of neuraminidase-treatment in studies of red cell aging. Blut 1981; 42: 95-98
    CrossrefPubMedSearch in Google Scholar
  • 21 Smedrod B, Aminof D. Studies on the Sequestration of chemically and enzymatically modified erythrocytes. Am J Hematol 1983; 15: 123-133
    CrossrefPubMedSearch in Google Scholar
  • 22 Harker LA. Platelet survival time. Its measurement and use. Prog Thromb Hemostas 1978; 4: 321-478
    PubMedSearch in Google Scholar
  • 23 Galili U, Clark MR, Shonet SB, Buehler J, Macher BA. Evolutionary relationship between natural anti-Gal antibody and Gal 1 → 3 Gal epitope in primates. Proc Natl Acad Sci USA 1987; 84: 1369-1373
    CrossrefPubMedSearch in Google Scholar
  • 24 Galili U. The natural anti-Gal antibody, the B-like antigen, and human red cell aging. Blood Cells 1988; 14: 205-220
    PubMedSearch in Google Scholar
  • 25 Schelepper-Schäffer J, Kolb-Bachoven V. Red cell ageing results in change in carbohydrate epitopes allowing for the recognition by galactose-specific receptors of rat liver macrophages. Blood Cells 1988; 14: 259-269
    PubMedSearch in Google Scholar