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Thromb Haemost 1993; 70(05): 834-837
DOI: 10.1055/s-0038-1649679
Platelets
Schattauer GmbH Stuttgart

Inhibitory Effect of Staphylokinase on Platelet Aggregation

Akira Suehiro
The Second Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
,
Yoshio Oura
The Second Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
,
Motoo Ueda
The Second Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
,
Eizo Kakishita
The Second Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
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Publication History

Received 18 March 1993

Accepted after revision 15 June 1993

Publication Date:
05 July 2018 (online)

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Summary

We investigated the effect of staphylokinase (SAK), which has specific thrombolytic properties, on human platelet aggregation. Platelet aggregation induced with collagen was observed following preincubation of platelets in platelet-rich plasma (PRP) or washed platelet suspension (WP) with SAK at 37° C for 30 min. SAK inhibited platelet aggregation in PRP only at the highest examined concentration (1 x 10-4 g/ml). Although SAK did not inhibit platelet aggregation in WP which contained fibrinogen, it did when the platelets had been preincubated with SAK and plasminogen. The most effective concentration in WP was 1 x 10-6 g/ml. The effect could be inhibited by adding aprotinin or α2-antiplasmin. The highest generation of plasmin in the same preincubation fluid was detected at 1 x 10-6 g/ml SAK. We concluded that SAK can inhibit platelet aggregation in WP by generating plasmin and/or fibrinogen degradation products, but is only partially effective in PRP because of the existence of α2-antiplasmin.

 

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