Aurintricarboxylic Acid Reduces Platelet Deposition in Stenosed and Endothelially Injured Rabbit Carotid Arteries more Effectively than other Antiplatelet Interventions

Palolo Golino; Massimo Ragni; Plinio Cirillo; Immacolata Pascucci; Michael D Ezekowitz; Aruna Pawashe; Annalisa Scognamiglio; Leonardo Pace; Angela Guarino; Massimo Chiariello

doi:10.1055/s-0038-1649858

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1995; 74(03): 974-979
DOI: 10.1055/s-0038-1649858

Original Article

Vessel Wall

Schattauer GmbH Stuttgart

Aurintricarboxylic Acid Reduces Platelet Deposition in Stenosed and Endothelially Injured Rabbit Carotid Arteries more Effectively than other Antiplatelet Interventions

Palolo Golino

The Department of Internal Medicine, Division of Cardiology, Yale University, New Haven, CT, USA

,

Massimo Ragni

The Department of Internal Medicine, Division of Cardiology, Yale University, New Haven, CT, USA

,

Plinio Cirillo

The Department of Internal Medicine, Division of Cardiology, Yale University, New Haven, CT, USA

,

Immacolata Pascucci

The Department of Internal Medicine, Division of Cardiology, Yale University, New Haven, CT, USA

,

Michael D Ezekowitz

¹The Department of Internal Medicine, Division of Cardiology, Yale University, New Haven, CT, USA

,

Aruna Pawashe

¹The Department of Internal Medicine, Division of Cardiology, Yale University, New Haven, CT, USA

,

Annalisa Scognamiglio

The Department of Internal Medicine, Division of Cardiology, Yale University, New Haven, CT, USA

,

Leonardo Pace

²The Division of Nuclear Medicine, 2nd School of Medicine, University of Naples, Italy

,

Angela Guarino

The Department of Internal Medicine, Division of Cardiology, Yale University, New Haven, CT, USA

,

Massimo Chiariello

The Department of Internal Medicine, Division of Cardiology, Yale University, New Haven, CT, USA

› Author Affiliations

Abstract

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Summary

In the present study we tested the effects of different antithrombotic interventions on platelet deposition in experimentally-stenotic rabbit carotid arteries with endothelial injury. Platelet deposition, quantitated by labeling autologous platelets with ₁₁₁ In-oxine, was significantly reduced compared to control animals by all interventions tested, i.e., R 68070, a drug with thromboxane A₂ synthase and receptor blocking properties, BN 52021, a PAF receptor antagonist, aurintricarboxylic acid (ATA), an inhibitor of platelet glycoprotein (Gp) Ib/von Wille-brand factor (vWf) interaction, AZ-1, a monoclonal antibody against rabbit GPIIb/IIIa, the platelet receptor for fibrinogen, and AP-1, a monoclonal antibody against rabbit tissue factor. ATA was significantly more effective than all the other interventions in reducing platelet deposition in the stenotic vessels. We conclude that inhibition of Gp Ib/vWf interaction may be a more suitable target for antithrombotic therapy under conditions of high shear stress, like those produced in this model.

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References

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