Identification and Genetic Analysis of a Common Molecular Variant of Histidine-rich Glycoprotein with a Difference of 2KD in Apparent Molecular Weight

B C Hennis; P A van Boheemen; S Wakabayashi; T Koide; J J M L Hoffmann; P Klevit; G Dooijewaard; J G Jansen; C Kluft

doi:10.1055/s-0038-1649971

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1995; 74(06): 1491-1496
DOI: 10.1055/s-0038-1649971

Original Articles

Fibrinolysis

Schattauer GmbH Stuttgart

Identification and Genetic Analysis of a Common Molecular Variant of Histidine-rich Glycoprotein with a Difference of 2KD in Apparent Molecular Weight

Autoren

B C Hennis

The Gaublus Laboratory TNO-PG, Leiden, The Netherlands
P A van Boheemen

The Gaublus Laboratory TNO-PG, Leiden, The Netherlands
S Wakabayashi

¹The Department of Life Science, Himeji Institute of Technology, Kamigorl, Japan
T Koide

¹The Department of Life Science, Himeji Institute of Technology, Kamigorl, Japan
J J M L Hoffmann

²The Haemostasis Division, Department of Clinical Laboratories, Catharina Hospital, Eindhoven, Leiden, The Netherlands
P Klevit

The Gaublus Laboratory TNO-PG, Leiden, The Netherlands
G Dooijewaard

The Gaublus Laboratory TNO-PG, Leiden, The Netherlands
J G Jansen

³The MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University Leiden, Leiden, The Netherlands
C Kluft

The Gaublus Laboratory TNO-PG, Leiden, The Netherlands

Abstract

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Summary

Two forms of histidine-rich glycoprotein (HRG) were detected on SDS-PAGE by silver staining and immunoblotting after isolation of the protein from pooled plasma using immuno-affinity chromatography followed by chromatography with heparin-Sepharose. Both forms were single-chain molecules and the apparent molecular weights of form 1 and form 2 were 77 kD and 75 kD respectively. Mendelian inheritance of both HRG forms was observed in four families with 24 informative meioses, strongly suggesting that the two forms are encoded by different alleles. The frequency of form 1 and form 2 in a group of 36 individuals was 0.35 and 0.65 respectively.

The difference between the two molecular variants was studied by direct sequence analysis of amplified exons of the HRG gene from 6 individuals who were homozygous either for form 1 or form 2. Five amino acid polymorphisms in three different exons were observed: Ile/Thr in exon 4; Pro/Ser in exon 5; His/Arg, Arg/Cys and Asn/Ile in exon 7. Analysis of these polymorphisms in 20 volunteers showed that only the Pro/Ser polymorphism at position 186 in exon 5 was coupled to the form of the HRG protein. Ser was found in form 1 and Pro in form 2. The presence of Ser at position 186 introduces a consensus sequence for a N-glycosylation site (Asn-X-Ser/Thr). By removing N-linked sugars with N-glycanase, it could be demonstrated that the difference between the two forms of HRG is caused by an extra carbohydrate group at Asn 184 in form 1.

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Referenzen

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