Thromb Haemost 1980; 43(01): 045-048
DOI: 10.1055/s-0038-1650009
Original Article
Schattauer GmbH Stuttgart

Inactivation of Human Factor VIII by Granulocyte Proteases

Katalin Váradi
The Department of Blood Coagulation, National Institute of Haematology and Blood Transfusion, Budapest, and Department of Biochemistry, University Medical School, Debrecen, Hungary
,
Katalin Marossy
The Department of Blood Coagulation, National Institute of Haematology and Blood Transfusion, Budapest, and Department of Biochemistry, University Medical School, Debrecen, Hungary
,
G Asbóth
The Department of Blood Coagulation, National Institute of Haematology and Blood Transfusion, Budapest, and Department of Biochemistry, University Medical School, Debrecen, Hungary
,
P Elödi
The Department of Blood Coagulation, National Institute of Haematology and Blood Transfusion, Budapest, and Department of Biochemistry, University Medical School, Debrecen, Hungary
,
Susan Elödi
The Department of Blood Coagulation, National Institute of Haematology and Blood Transfusion, Budapest, and Department of Biochemistry, University Medical School, Debrecen, Hungary
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Publikationsverlauf

Received 18. Oktober 1979

Accepted 04. Januar 1980

Publikationsdatum:
13. Juli 2018 (online)

Summary

The effect of human granulocyte proteases and related pancreatic enzymes on the procoagulant (VIII:C) and the von Willebrand (VIIIR:WF) activities of factor VIII was investigated. VIII:C appeared to be several hundred times more sensitive towards granulocyte enzymes than VIIIR: WF. The elastase-like protease (ELP) decreased both activities of factor VIII more effectively than the chymotrypsin-like enzyme (CLP). ELP increased co-operatively the inactivating effect of CLP. In the presence of a CLP-ELP mixture in a ratio of 3:1, the rate of inactivation of VIII:C was seven times as high as that with CLP alone.

Factor VIII was more resistant towards the damaging effect of pancreatic enzymes. The rate of inactivation of VIII: C was about a thousand times and that of VIIIR:WF about a hundred times lower than those measured with the related granulocyte enzymes of the same proteolytic activity. The sensitivity of VIII: C towards proteolysis was not as pronounced with pancreatic enzymes as with granulocyte proteases.

Our data suggest that, even though CLP and ELP are called "-like" enzymes, the specificity of granulocyte proteases is not identical with that of the pancreatic enzymes. The extreme sensitivity of VIII: C for granulocyte proteases may bring about blood coagulation disorders in certain pathological conditions.

 
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