Effects of Dimethyl Sulphoxide and of Vitamin A on Human Platelets

M. G Davey; E. F Lüscher

doi:10.1055/s-0038-1650204

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1968; 19(01/02): 012-017
DOI: 10.1055/s-0038-1650204

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Effects of Dimethyl Sulphoxide and of Vitamin A on Human Platelets

M. G Davey^*

¹Theodor Kocher Institute, University of Berne

,

E. F Lüscher

¹Theodor Kocher Institute, University of Berne

› Author Affiliations

Abstract

Summary

Dimethyl sulphoxide (DMSO) causes some release of amino acids, adenine nucleotides, and acid phosphatase and β-glucuronidase activities from washed human platelets. Addition of vitamin A with DMSO greatly enhances the effect; but vitamin A in ethanol added to platelets fails to release amino acids and nucleotides. Release appears to be due to a change in permeability of the platelet membrane, not to the discharge of contents of intracellular structures. Lipid peroxidation of membrane phospholipids, or a similar reaction, may be involved. Thrombin causes a different type of alteration in platelet permeability.

The results suggest that DMSO should not be used as a preservative for platelets. They may have some bearing on the mechanisms of toxicity of DMSO and vitamin A.

Full Text

References

References
1 Djerassi I, Roy A. A method for preservation of viable platelets: combined effects of sugars and dimethylsulfoxide. Blood 1963; 22: 703
2 Djerassi I, Farber S, Roy A, Cavins J. Preparation and in vivo circulation of human platelets preserved with combined dimethylsulfoxide and dextrose. Transfusion 1966; 6: 572
3 Dingle J.T. Action of vitamin A on the stability of lysosomes in vivo and in vitro. de Reuck A. V, Cameron S, Cameron M. P. @@London: In: Ciba Foundation Symposium on Lysosomes. Edit 1963; 384
4 Lucy J.A, Luscombe M, Dingle J.T. Studies on the mode of action of excess of vitamin A. 8. Mitochondrial swelling. Biochem. J. 1963; 89: 419
5 Lucy J.A, Dingle J.T. Fat-soluble vitamins and biological membranes. Nature (Lond.). 1964; 204: 156
6 Woodin A.M, Wienege A.A. The participation of calcium, adenosine triphosphate and adenosine triphosphatase in the extrusion of the granule proteins from the polymorphonuclear leucocyte. Biochem. J. 1964; 90: 498
7 Kowalski E, Kopeć M, Węgrzynowicz Z, Hurwic M, Budzyński A.Z. A lysosomal concept of the platelet release reaction and viscous metamorphosis. Thrombos. Diathes. haemorrh. (Stuttg.) 1966; 16: 134
8 Bettex-Galland M, Lüscher E.F. Studies on the metabolism of human blood platelets in relation to clot retraction. ibid 1960; 4: 178
9 Davey M.G, Lüscher E.F. Actions of some coagulant snake venoms on blood platelets. Nature (Lond.) 1965; 207: 730
10 Siegel A.M, Lüscher E.F. Non-identity of the α-granules of human blood platelets with typical lysosomes. Nature (Lond.) 1967; 215: 745
11 Davey M.G, Lüscher E.F. Unpublished.
12 Weissmann G, Sessa G, Bevans V. Effect of DMSO on the stabilization of lysosomes by cortisone and chloroquine in vitro. Ann. N. Y. Acad. Sci. 1967; 141: 326
13 MacGregor W.S. The chemical and physical properties of DMSO. ibid. 1967; 141: 3
14 Muset P.P, Martin-Estève J. Physiological cell permeability and pharmacological action of DMSO. Experientia (Basel) 1965; 21: 649
15 Robinson J.D. Interaction between protein sulphydryl groups and lipid double bonds in biological membranes. Nature (Lond.) 1966; 212: 199
16 Gerhards E, Gibian H. The metabolism of dimethyl sulfoxide and its metabolic effects in man and animals. Ann. N. Y. Acad. Sci. 1967; 141: 65
17 Preston J.W, Bouchier I.A.D. Potentiation of carbon tetrachloride toxicity by dimethyl sulphoxide.. Nature (Lond.) 1967; 214: 734
18 Baccino F.M, Rita G.A, Dianzani M.U. Further experiments on the action of CCl₄ on lysosomes in vitro. Enzymologia 1965; 29: 169
19 Comporti M, Saccocci C, Dianzani M.U. Effect of CCl₄ in vitro and in vivo on lipid peroxidation of rat liver homogenates and subcellular JT fractionsibid. 1965; 29: 185
20 de Duve C, Pressman B.C, Gianetto R, Wattiaux R, Appelmans F. Tissue fractionation studies. 6. Intercellular distribution patterns of enzymes in rat-liver tissue. Biochem. J. 1955; 60: 604
21 Buckingham S, Maynert E.W. The release of 5-hydroxytryptamine, potassium and amino acids from platelets. J. Pharmacol. (Baltimore) 1964; 143: 332
22 Cousin C, Vainer H, Michel H, Gautier A, Caen J. Enzymes et fonctions des plaquettes au cours de leur conservation. Nouv. Rev. franc. Hémat. 1966; 6: 279
23 Cohen P, Gardner F.H. Platelet preservation II. Preservation of canine platelet concentrates by freezing in solutions of glycerol-plasma. J. clin. Invest. 1962; 41: 10
24 Smith E.R, Hadidian Z, Mason M.M. The single - and repeated — dose toxicity of dimethyl sulfoxide. Ann. N. Y. Acad. Sci. 1967; 141: 96
25 Caujolle F.M.E, Caujolle D.H, Gros S.B, Calvet M.J. Limits of toxic and teratogenic tolerance of dimethyl sulfoxide. ibid. 1967; 141: 110
26 Jacob S.W, Bischel M, Herschler R.J. Dimethyl sulfoxide: Effects on the permeability of biologic membranes (preliminary report). Curr. ther. Res. 1964; 6: 193