Download PDF
Thromb Haemost 1996; 75(01): 175-181
DOI: 10.1055/s-0038-1650239
Original Article
Schattauer GmbH Stuttgart

Epinephrine Augments Platelet Recruitment to Immobilized Collagen in Flowing Blood - Evidence for a von Willebrand Factor-mediated Mechanism

Pirjo Mustonen
The Wihuri Research Institute, Helsinki, Finland
,
Riitta Lassila
The Wihuri Research Institute, Helsinki, Finland
› Author Affiliations
Further Information

Publication History

Received 28 April 1995

Accepted after resubmission 19 September 1995

Publication Date:
10 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

Although elevated plasma epinephrine (epi) levels are associated with clinical atherothrombosis, the role of epi in platelet-vessel wall interaction has not been established. Our aim was to study the effect of high physiological epi (10 nM) in an experimental model which tests the interaction between platelets and immobilized collagen in whole blood. Shear forces and anticoagulation were modulated. Epi significantly enhanced platelet deposition, but only at high shear rate (1,600 s-1)- In PPACK- or LMWH-anticoagulated blood, the increase in platelet deposition was 32 to 85 % (p <0.02-0.05). Furthermore, platelet aggregation was cotriggered with subthreshold concentrations of epi and ristocetin, and monoclonal antibodies against glycoprotein (GP) lb (AN 51 and SZ 2) attenuated epi-induced aggregation. We conclude that epi is capable of augmenting platelet functions, which are dependent on the interaction of vWF with GP lb and GP IIb/IIIa. Via this mechanism epi may promote arterial thrombosis in vivo.

 

  • References

  • 1 Hauss WH, Bauch H-J, Schulte H. Adrenaline and noradrenaline as possible chemical mediators in the pathogenesis of arteriosclerosis. Ann N Y Acad Sci 1990; 598: 091-101
    CrossrefPubMedSearch in Google Scholar
  • 2 Schafi S, Cusack NJ, Born GVR. Increased uptake of methylated low-den-sity lipoprotein induced by noradrenaline in carotid arteries of anaesthetized rabbits. Proc R Soc Lond B 1989; 235: 289-298
    CrossrefPubMedSearch in Google Scholar
  • 3 Krukeja RS, Datta BN, Charakavarti RN. Catecholamine-induced aggravation of aortic and coronary atherosclerosis in monkeys. Atherosclerosis 1981; 40: 291-298
    CrossrefPubMedSearch in Google Scholar
  • 4 Folts JD, Rowe GG. Epinephrine potentiation of in vivo stimuli reverses aspirin inhibition of platelet thrombus formation in stenosed canine coronary arteries. Thromb Res 1988; 50: 507-516
    CrossrefPubMedSearch in Google Scholar
  • 5 Roux SP, Sakariassen KS, Turitto VT, Baumgartner HR. Effect of aspirin and epinephrine on experimentally induced thrombogenesis in dogs, a parallelism between in vivo and ex vivo thrombosis models. Arterioscler Thromb 1991; 11: 1182-1191
    CrossrefPubMedSearch in Google Scholar
  • 6 Raab W. Hormonal and neurogenic cardiovascular disorders. Endocrine and neuro-endocrine factors in pathogenesis and treatment Raab W. ed Williams and Wilkins Co; Baltimore: 1953: 73-90
    Search in Google Scholar
  • 7 Ardlie NG, McGuiness JA, Garret JJ. Effect on human platelets of catecholamines at levels achieved in the circulation. Atherosclerosis 1985; 58: 251-259
    CrossrefPubMedSearch in Google Scholar
  • 8 Hjemdahl P, Chronos NAF, Wilson DJ, Bouloux P, Goodall AH. Epinephrine sensitizes human platelets in vivo and in vitro as studied by fibrinogen binding and p-selectin expression. Arterioscler Thromb 1994; 14: 77-84
    CrossrefPubMedSearch in Google Scholar
  • 9 Larsson PT, Hjemdahl P, Olsson G, Egberg N, Hornstra G. Altered platelet function during mental stress and adrenaline infusion in humans: evidence for an increased aggregability in vivo as measured by filtragometry. Clin Sci 1989; 76: 369-376
    CrossrefPubMedSearch in Google Scholar
  • 10 Goto S, Ikeda Y, Murata M, Handa M, Takahashi E, Yoshioka A, Fujimura Y, Fukuyama M, Handa S, Ogawa S. Epinephrine augments von Willebrand factor-dependent shear-induced platelet aggregation. Circulation 1992; 86: 1859-1863
    CrossrefPubMedSearch in Google Scholar
  • 11 Jouce DA, Beilin LJ, Vandongen R, Davidson L. Epinephrine sulfation in the forearm: arteriovenous differences in free and conjugated catecholamines. Life Sci 1982; 31: 2513-2517
    CrossrefPubMedSearch in Google Scholar
  • 12 Collen D, Matsuo O, Stassen JM, Kettner C, Shaw E. In vivo studies of a synthetic inhibitor of thrombin. J Lab Clin Med 1982; 99: 76-83
    PubMedSearch in Google Scholar
  • 13 Lozano M, Bos A, de Groot PhG, van Willigen G, Meuleman DG, Ordinas A, Sixma JJ. Suitability of low-molecular-weight heparin(oid)s and a pentasaccharide for an in vitro human thrombosis model. Arterioscler Thromb 1994; 14: 1215-1222
    CrossrefPubMedSearch in Google Scholar
  • 14 Timmons S, Hawiger J. Isolation of human platelets by albumin gradient and gelfiltration. In: Platelets, Receptors, Adhesion, Secretion Hawiger J. ed Methods in Enzymology. Academic Press San Diego; CA: 1989: 11-22
  • 15 Williams BR, Gelman RA, Poppke DC, Piez KA. Collagen fibril formation: optimal in vitro conditions and preliminary kinetic results. J Biol Chem 1978; 253: 6578-6585
    PubMedSearch in Google Scholar
  • 16 Holmes DF, Capaldi MJ, Chapman JA. Reconstitution of collagen fibrils in vitro; the assembly process depends on the initiating procedure. Int J Biol Macromol 1986; 8: 161-166
    CrossrefPubMedSearch in Google Scholar
  • 17 Badimon L, Badimon JJ, Galvez A, Fuster V. Influence of arterial damage and wall shear rate on platelet deposition. Arteriosclerosis 1986; 6: 312-320
    CrossrefPubMedSearch in Google Scholar
  • 18 Holmsen H, Dangelmaier CA. Measurement of secretion of serotonin. In: Platelets: Receptors, Adhesion, Secretion Hawiger J. ed Methods in Enzymology. Academic Press San Diego; CA: 1989: 205-210
  • 19 Tuszynski GP, Murphy A. Spectrophotometric quantitation of anchorage-dependent cell numbers using the bicinchonic acid protein assay reagent. AnalBiochem 1990; 184: 189-191
    PubMedSearch in Google Scholar
  • 20 Sakariassen KS, Baumgartner HR. Axial dependence of platelet-collagen interactions in flowing blood. Upstream thrombus growth impairs downstream platelet adhesion. Arteriosclerosis 1989; 9: 33-42
    CrossrefPubMedSearch in Google Scholar
  • 21 Bolton AE, Hunter WM. The labeling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochemistry 1973; 133: 529-539
    CrossrefPubMedSearch in Google Scholar
  • 22 Keraly CL, Kinlough-Rathbome R, Packham MA, Suzuki H, Mustard JF. Conditions affecting the responses of human platelets to epinephrine. Thromb Haemost 1988; 60: 209-216
    Thieme ConnectPubMedSearch in Google Scholar
  • 23 Lanza F, Beretz A, Stierle A, Hanau D, Kubina M, Cazenave J-P. Epinephrine potentiates human platelet activation but is not an aggregating agent. Am J Physiol 1988; 255: H1276-H1288
    PubMedSearch in Google Scholar
  • 24 Shattil SJ, Budzynski A, Scrutton MC. Epinephrine induces platelet fibrinogen receptor expression, fibrinogen binding, and aggregation in whole blood in the absence of other excitatory agonists. Blood 1989; 73: 150-158
    PubMedSearch in Google Scholar
  • 25 Steen VM, Holmsen H, Aarbakke G. The platelet-stimulating effect of adrenaline through α2-adrenergic receptors require simultaneous activation by a true stimulatory platelet agonist. Thromb Haemost 1993; 70: 506-513
    Thieme ConnectPubMedSearch in Google Scholar
  • 26 Valles J, Santos MT, Aznar J, Marcus AJ, Martinez-Sales V, Portoles M, Broekman MJ, Safier LB. Erythrocytes metabolically enhance collagen-induced platelet responsiveness via increased thromboxane production, adenosine diphosphate release, and recruitment. Blood 1991; 78: 154-162
    CrossrefPubMedSearch in Google Scholar
  • 27 Ikeda Y, Handa M, Kawano K, Kamata T, Murata M, Araki Y, Anbo H, Kawai Y, Watanabe K, Itagaki I, Sakai K, Ruggeri ZM. The role of von Willebrand factor and fibrinogen in platelet aggregation under varying shear stress. J Clin Invest 1991; 87: 1234-1240
    CrossrefPubMedSearch in Google Scholar
  • 28 Sakariassen K, Bolhuis PA, Sixma JJ. Human platelet adhesion to artery subendothelium is mediated by factor VIII-von Willebrand factor bound to the endothelium. Nature 1979; 279: 636-638
    CrossrefPubMedSearch in Google Scholar
  • 29 Fressinaud E, Federici AB, Castaman G, Rothschild C, Rodeghiero F, Baumgartner HR, Manucci PM, Meyer D. The role of platelet von Willebrand factor in platelet adhesion and thrombus formation: a study of 34 patients with various subtypes of type I von Willebrand disease. Br J Haematol 1994; 86: 327-332
    CrossrefPubMedSearch in Google Scholar
  • 30 Fressinaud E, Baruch D, Girma J-P, Sakariassen KS, Baumgartner HR, Meyer D. Von Willebrand factor-mediated platelet adhesion to collagen involves platelet membrane glycoprotein IIb-IIIa as well as glycoprotein Ib. J Lab Clin Med 1988; 112: 58-67
    PubMedSearch in Google Scholar
  • 31 Ruggeri ZM. Mechanisms of shear-induced platelet adhesion and aggregation. Thromb Haemost 1993; 70: 119-123
    Thieme ConnectPubMedSearch in Google Scholar
  • 32 Alevriadou BR, Moake JL, Turner NA, Ruggeri ZM, Folie BJ, Phillips MD, Schreiber AB, Hrinda ME, McIntire LV. Real-time analysis of shear-dependent thrombus formation and its blockade by inhibitors of von Willebrand factor binding to platelets. Blood 1993; 81: 1263-1276
    PubMedSearch in Google Scholar
  • 33 Kunicki TJ, Orchekowski R, Annis D, Honda Y. Variability of integrin α2β1 activity on human platelets. Blood 1993; 82: 2693-2703
    PubMedSearch in Google Scholar
  • 34 Turitto V, Weiss HJ, Baumgartner HR. Rheological factors influencing platelet interaction with vessel surfaces. J Rheol 1979; 23: 735-739
    CrossrefPubMedSearch in Google Scholar
  • 35 Santoro SA. Identification of a 160,000 D platelet membrane protein that mediates the initial divalent cation-dependent adhesion of platelets to collagen. Cell 1986; 46: 913-920
    CrossrefPubMedSearch in Google Scholar
  • 36 Chow TW, Heliums JD, Schafer AI, Kroll MH. Epinephrine potentiates the interaction between von Willebrand factor and platelet membrane glycoprotein Ib. Thromb Haemost 1993; 69: 601 (Abstr.)
    PubMedSearch in Google Scholar
  • 37 Cattaneo M, Mustard JF, Canciani MT, Richardson M, Federici AB, Mannucci PM. Conditions influencing the interaction of asialo von Willebrand factor with human platelets - the effects of external ionized calcium concentration and the role of arachidonate pathway. Thromb Haemost 1988; 60: 280-288
    Thieme ConnectPubMedSearch in Google Scholar
  • 38 Michelson AD, Benoit SE, Kroll MH, Li J-M, Rohrer MJ, Kestin AS, Barnard MR. The activation-induced decrease in the platelet surface expression of the glycoprotein Ib-IX complex is reversible. Blood 1994; 83: 3562-3573
    PubMedSearch in Google Scholar
  • 39 Ruggeri ZM. The platelet glycoprotein Ib-IX complex. In: Progress in Hemostasis and Thrombosis Coller BS. ed WB Saunders Company; Philadephia, PA: 1991: 35-68
  • 40 Eyster ME, Ballard JO, Prager D. Comparison of factor VIII levels after adrenalin in classic hemophilia and von Willebrand’s disease. Thromb Haemost 1978; 39: 657-662
    Thieme ConnectPubMedSearch in Google Scholar
  • 41 Jern C, Eriksson E, Tengbom L, Risberg B, Wadenvik H, Jern S. Changes of plasma coagulation and fibrinolysis in response to mental stress. Thromb Haemost 1989; 62: 767-771
    Thieme ConnectPubMedSearch in Google Scholar