Characterization of a cDNA Encoding Murine Coagulation Factor VII

Esohe Idusogie; Elliot Rosen; Jie-Ping Geng; Peter Carmeliet; Desirá Collen; Francis J Castellino

doi:10.1055/s-0038-1650301

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1996; 75(03): 481-487
DOI: 10.1055/s-0038-1650301

Original Article

Schattauer GmbH Stuttgart

Characterization of a cDNA Encoding Murine Coagulation Factor VII

Esohe Idusogie

¹The Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA

,

Elliot Rosen

¹The Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA

²The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium

,

Jie-Ping Geng

¹The Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA

,

Peter Carmeliet

²The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium

,

Desirá Collen

²The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium

,

Francis J Castellino

¹The Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA

› Institutsangaben

Abstract

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Summary

The cDNA encoding murine coagulation factor VII (mfVII) was isolated and reconstructed from a Λ Zap cDNA library generated from murine liver mRNA. The cDNA contains 1903 nucleotides spanning 15 bases upstream of the 5’-translation initiation codon, an open reading frame of 1338 nucleotides, 550 nucleotides downstream of the first stop codon and a 3’ poly(A) tail. The translation product is composed of a 41-amino acid signal/propeptide region followed by a 405-residue mature protein. The latter is highly homologous to that of human, rabbit, bovine, Rhesus monkey, and canine fVII. All protein domains of hfVII are strictly conserved in mfVII.

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Referenzen

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