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Thromb Haemost 1996; 75(04): 655-660
DOI: 10.1055/s-0038-1650338
Original Article
Schattauer GmbH Stuttgart

Porcine von Willebrand Factor Binding to Human Platelet GPIb Induces Transmembrane Calcium Influx

Mario Mazzucato
The Servizio Immunotrasfusionale e Analisi Cliniche, I.R.C.C.S., C.R.O., Aviano, PN, Italy
,
Luigi De Marco
The Servizio Immunotrasfusionale e Analisi Cliniche, I.R.C.C.S., C.R.O., Aviano, PN, Italy
,
Paola Pradella
The Servizio Immunotrasfusionale e Analisi Cliniche, I.R.C.C.S., C.R.O., Aviano, PN, Italy
,
Adriana Masotti
The Servizio Immunotrasfusionale e Analisi Cliniche, I.R.C.C.S., C.R.O., Aviano, PN, Italy
,
Francesco I Pareti
1   A. Bianchi Bonomi Hemophilia and Thrombosis Center, I.R.C.C.S., Maggiore Hospital, University of Milano, Italy
› Author Affiliations
Further Information

Publication History

Received 15 May 1995

Accepted after resubmission 18 December 1995

Publication Date:
10 July 2018 (online)

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Summary

Porcine von Willebrand factor (P-vWF) binds to human platelet glycoprotein (GP) lb and, upon stirring (1500 rpm/min) at 37° C, induces, in a dose-dependent manner, a transmembrane flux of Ca2+ ions and platelet aggregation with an increase in their intracellular concentration. The inhibition of P-vWF binding to GP lb, obtained with anti GP lb monoclonal antibody (LJ-Ib1), inhibits the increase of intracellular Ca2+ concentration ([Ca2+]i) and platelet aggregation. This effect is not observed with LJ-Ib10, an anti GP lb monoclonal antibody which does not inhibit the vWF binding to GP lb. An anti GP Ilb-IIIa monoclonal antibody (LJ-CP8) shown to inhibit the binding of both vWF and fibrinogen to the GP IIb-IIIa complex, had only a slight effect on the [Ca2+]i rise elicited by the addition of P-vWF. No inhibition was also observed with a different anti GP IIb-IIIa monoclonal antibody (LJ-P5), shown to block the binding of vWF and not that of fibrinogen to the GP IIb-IIIa complex. PGE1, apyrase and indomethacin show a minimal effect on [Ca2+]i rise, while EGTA completely blocks it. The GP lb occupancy by recombinant vWF fragment rvWF445-733 completely inhibits the increase of [Ca2+]i and large aggregates formation. Our results suggest that, in analogy to what is seen with human vWF under high shear stress, the binding of P-vWF to platelet GP lb, at low shear stress and through the formation of aggregates of an appropriate size, induces a transmembrane flux of Ca2+, independently from platelet cyclooxy-genase metabolism, perhaps through a receptor dependent calcium channel. The increase in [Ca2+]i may act as an intracellular message and cause the activation of the GP IIb-IIIa complex.

 

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