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DOI: 10.1055/s-0038-1650339
Characterization of Fcγ Receptors on Human Megakaryocytes
Publikationsverlauf
Received 21. Juni 1995
Accepted after resubmission 18. Dezember 1995
Publikationsdatum:
10. Juli 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/199604/lookinside/thumbnails/10-1055-s-0038-1650339-1.jpg)
Summary
Megakaryocyte and platelet Fcγ receptors (FcR) are of importance in the pathophysiology of immune complex-mediated thrombocytopenias such as heparin-induced thrombocytopenia. In this study, FcγR proteins and mRNAs in normal human megakaryocytes were examined. FcγR proteins were studied with immunocytochemical staining, dual colour flow cytometry and immunoprecipitation using monoclonal antibodies against FcγR I, FcγR II and FcγR III. FcγR mRNAs were measured with biotinylated cDNA or oligonucleotide probes using a novel quantitative in situ hybridization technique. Using these techniques, FcγR II protein and mRNA, but not FcγR I and FcγR III proteins and transcripts were detected in megakaryocytes. Further, transcript analysis showed that megakaryocytes contain only the transcript of FcγR IIA gene but no transcripts of FcγR IIB nor FcγR IIC genes; FcγR IIA transcripts with and without the transmembrane (TM) exon are present in approximately equal proportions. In contrast, neutrophils and macrophages also contain FcγR IIA transcript but FcγR IIA transcript with the TM exon predominates suggesting cell lineage-specific FcγR IIA expression. FcγR IIA transcript lacking the TM exon predicts the presence of a potential soluble form of FcγR in platelets and megakaryocytes which may have a physiological role as it can compete with the membrane-bound FcγR IIA for binding of IgG-containing immune complexes and thus protect these cells from excessive binding and injurious effects of immune complexes.
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