Fibrinogen Promotes Adhesion of Monocytic to Human Mesothelioma Cells

Summary

Adhesion between monocytic and mesothelioma or pleural meso-thelial cells influences stromal remodeling in pleural neoplasia. We found that cultured monocytic cells (U937) adhere to either human pleural mesothelioma (MS-1) or mesothelial (MeT5A) cells in vitro. ¹²⁵I-fibrinogen bound specifically and saturably to either cell line, and specific fibrinogen binding increased upon stimulation of these cells with proinflammatory agents such as phorbol myristate (PMA), lipo-polysaccharide (LPS) or tumor necrosis factor (TNF-±). We purified the fibrinogen receptor protein from a membrane fraction of MS-1 cells and identified it by immunoprecipitation as intercellular adhesion molecule (ICAM-1). Anti-ICAM-1 antibody or antisense oligonucleotides inhibited fibrinogen-mediated cell adhesion and binding of ¹²⁵I-fibrinogen to mesothelioma or mesothelial cells. Cultured monocytic cells adhere to either mesothelioma or mesothelial cells, and the interaction is promoted by fibrinogen binding ICAM-1 at the cell surface. ICAM-1 is expressed by mesothelioma cells and CD 1 lb by macrophages in the fibrinous mesothelioma tumor stroma. The data suggest a common mechanism by which monocytic cells could adhere to either malignant mesothelioma cells or the mesothelial surface in pleural neoplasia.

• References

• 1 Springer TA. Adhesion receptors of the immune system. Nature 1990; 346: 425-434
• 2 Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell 1992; 69: 11-25
• 3 Sahn S. The pleura. Am Rev Resp Dis 1988; 138: 184-234
• 4 Craighead JE, Kane AB. The pathogenesis of malignant and nonmalignant serosal lesion in body cavities consequent to asbestos exposure. In: Lung Biology in Health and Disease Jaurand M-C, Bignon J, (eds) Lenfant C. (ex. ed) Marcel Dekker; NY: 1994. Vol 78 79-100
• 5 Jonjic N, Peri G, Bemasconi S, Sciacca FL, Colatta F, Pelicci PG, Lanfrancone L, Manovani A. Expression of adhesion molecules and chemotactic cytokines in cultured human mesothelial cells. J Exp Med 1992; 176: 1165-1174
• 6 Languino LR, Plescia J, Duperray A, Brian AA, Plow EF, Geltosky JE, Altieri DC. Fibrinogen mediates leukocyte adhesion to vascular endothelium through an ICAM-1-dependent pathway. Cell 1993; 73: 1423-1434
• 7 Hsu SU, Hsu PL, Zhao X, Kao-Shan CS, Whang-Peng J. Establishment of human mesothelioma cell lines (MS-1, -2) and production of a monoclonal antibody (anti-MS) with diagnostic and therapeutic potential. Cancer Res 1988; 48: 5228-5236
• 8 Idell S, Zweib C, Kumar A, Koenig KB, Johnson AR. Pathways of fibrin turnover of human pleural mesothelial cells in vitro. Am J Cell Mol Biol 1992; 7: 414-426
• 9 Brown LF, Lanir N, McDonagh J, Tognazzi K, Dvorak AM, Dvorak HF. Fibroblast migration in fibrin gel matrices. Am J Pathol 1993; 142: 273-283
• 10 Fraker PJ, Speck Jr JC. Protein and cell membrane iodinations with a sparingly soluble chloramine 1,3,4,6-tetrachloro-3a, 6a diphenylglycoluril. Biochem Res Comm 1978; 80: 849-857
• 11 Shetty S, Kumar A, Johnson AR, Idell S. Regulation of mesothelial cell mitogenesis by antisense oligonucleotides for the urokinase receptor. Antisense Res and Devel 1995; 5: 307-314
• 12 Idell S, Pueblits S, Emre S, Gungen Y, Gray L, Kumar A, Holiday D, Koenig KB, Johnson AR. Regulation of fibrin deposition by malignant mesothelioma. Am J Pathol 1995; 147: 1318-1329
• 13 Altieri DC, Bader R, Mannucci PM, Edgington TM. Oligospecificity of the cellular adhesion receptor MAC-1 encompasses an inducible recognition specificity for fibringen. J Cell Biol 1988; 107: 1893-1900
• 14 Dvorak H. Tumors: wounds that do not heal. N Engl J Med 1982; 315: 1650-1659
• 15 Dvorak HF, Senger DR, Dvorak AM. Fibrin as a component of the tumor stroma: origins and biologic significance. Cancer Met Rev 1983; 2: 41-73
• 16 Boutin C, Viallat JR, Rey F, Astoul P. Clinical diagnosis of pleural malignancies. In: Lung Biology in Health and Disease Jaurand MC, Bignon J, (eds) Lenfant C. (ex. ed.) Marcel Dekker; NY: Vol 78 1994: 71-78
• 17 Bignon J, Gee JB. Pleural fibrogenesis. The Pleura in Health and Disease Marcel Dekker; NY: 1985: 417-443
• 18 Wilcox M, Leptin M. Tissue-specific modulation of a set of related cell surface antigens in drosophila. Nature 1985; 316: 351-354
• 19 Menko SA, Boettiger D. Occupation of the extracellular matrix receptor, integrin, is a control point for myogenic differentiation. Cell 1987; 51: 51-58
• 20 Dicorleto PE, Motte CA. Thrombin causes increased monocytic-cell adhesion to endothelial cells through a protein kinase C-dependent pathway. Biochem J 1989; 264: 71-77
• 21 Li R, Nortamo P, Kantor C, Kovanen P, Timonen T, Gahmberg CG. A leukocyte integrin binding peptide from intercellular adhesion molecule-2 stimulates T cell adhesion and natural killer cell activity. J Biol Chem 1993; 268: 21474-21477
• 22 Diamond MS, Stauton DE, Marlin SD, Springer TA. Binding of the integrin Mac-1 (CD 1 lb/CD 18) to the third immunoglobulin-like domain of ICAM-1 (CD54) and its regulation by glycosylation. Cell 1991; 65: 961-971
• 23 Amaout MA. Structure and function of leukocyte adhesion molecules CD11/CD 18. Blood 1990; 75: 1037-1050
• 24 Paltarrryo M, Prieto J, Rincon J, Timonen T, Lundberg C, Lindbom L, Asjo B, Gahmberg CG. Leukocyte-cell adhesion: a molecular process fundamental in leukocyte physiology. Immunol Rev 1990; 114: 67-108
• 25 Clark EA, Ledbetter JA, Holly PS, Dinndorf G, Schu G. Polypeptides on human B lymphocytes associated with cell activation. Hum Immunol 1986; 16: 1000-1105
• 26 Bevilaqua MP, Pober JS, Wheeler ME, Cotran RS, Gimbrone MA. Interleukin 1 acts on cultured human endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes and related cell lines. J Clin Invest 1985; 76: 2003-2011
• 27 Pober JS, Gimbrone MA, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA. Overlapping patterns of activation of human endothelial cells by interleukin-1, tumor necrosis factor and immune interferon. J Immunol 1986; 137: 1893-1896
• 28 Levesque JP, Hatzfeld A, Hatzfeld J. Fibrinogen mitogenic effect on hematopoietic cell lines: control via receptor modulation Proc Natl Acad Sci USA 1986; 83: 6494-6498