Macrophages and Neutrophils Infiltrating into the Liver Are Responsible for Tissue Factor Expression in a Rabbit Model of Acute Obstructive Cholangitis

Aiichiro Higure; Kohji Okamoto; Keiji Hirata; Hidekazu Todoroki; Yukihisa Nagafuchi; Shigeaki Takeda; Hidenori Katoh; Hideaki Itoh; Keiichi Ohsato; Shin Nakamura

doi:10.1055/s-0038-1650368

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1996; 75(05): 791-795
DOI: 10.1055/s-0038-1650368

Original Article

Schattauer GmbH Stuttgart

Macrophages and Neutrophils Infiltrating into the Liver Are Responsible for Tissue Factor Expression in a Rabbit Model of Acute Obstructive Cholangitis

Aiichiro Higure

¹The Department of Surgery-1, University of Occupational and Environmental Health, Kitakyushu

,

Kohji Okamoto

¹The Department of Surgery-1, University of Occupational and Environmental Health, Kitakyushu

,

Keiji Hirata

¹The Department of Surgery-1, University of Occupational and Environmental Health, Kitakyushu

,

Hidekazu Todoroki

¹The Department of Surgery-1, University of Occupational and Environmental Health, Kitakyushu

,

Yukihisa Nagafuchi

¹The Department of Surgery-1, University of Occupational and Environmental Health, Kitakyushu

,

Shigeaki Takeda

¹The Department of Surgery-1, University of Occupational and Environmental Health, Kitakyushu

,

Hidenori Katoh

¹The Department of Surgery-1, University of Occupational and Environmental Health, Kitakyushu

,

Hideaki Itoh

¹The Department of Surgery-1, University of Occupational and Environmental Health, Kitakyushu

,

Keiichi Ohsato

¹The Department of Surgery-1, University of Occupational and Environmental Health, Kitakyushu

,

Shin Nakamura

²The Department of Molecular and Cellular Biology, Primate Research Institute, Kyoto University, Japan

› Author Affiliations

Abstract

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Summary

Acute obstructive cholangitis (AOC) is one of the most fatal outcomes in sepsis, and frequently complicates disseminated intravascular coagulation (DIC). Recently we found that the plasma tissue factor (TF) level increased and changed in parallel with plasma markers of DIC in patients with AOC. To elucidate the role of TF in the pathogenesis of coagulopathy in AOC, we investigated the plasma levels of TF and its localization by immunohistochemical staining in rabbit models of AOC. Plasma TF activity significantly increased 3 h after the insult (0.63 ± 0.19 U/ml; p <0.01) compared with that beforehand (0.05 ± 0.02 U/ml), then reached a maximum level at 6 h (0.94 ±0.16 U/ml). The fluctuations in plasma TF activity correlated with those of the coagulation parameters including platelet count, fibrinogen, prothrombin time, and antithrombin III activity. Immunohistochemically, enhanced expression of TF was mainly detected in macrophages and neutrophils that had infiltrated into the liver sinusoids and around the bile duct, but not in the sinusoidal endothelial cells. A double immunofluorescence study revealed the concomitant presence of TF and fibrin at sites where macrophages and neutrophils had conglomerated. However, we could not detect an apparent change in TF expression in the lung or kidney. These data suggest that macrophages and neutrophils infiltrating into the liver sinusoids and around the bile duct play a pivotal role in TF expression, leading to coagulopathy in the acute phase of obstructive cholangitis in rabbits.

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References

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