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DOI: 10.1055/s-0038-1650399
N-3 Fatty Acid-induced Lipid Peroxidation in Human Platelets is Prevented by Catechins
Publikationsverlauf
Received 21. November 1995
Accepted after resubmission 06. März 1996
Publikationsdatum:
11. Juli 2018 (online)
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Summary
The effects of catechins were studied in both unstimulated platelets and platelets submitted to a mild oxidative stress induced by docosa-hexaenoic acid (DHA). Human unstimulated platelets were incubated in the presence of catechin or epicatechin (1, 10 or 100 μM) for 2 h at 37° C. Treatment by 1 μM epicatechin was sufficient to induce a significant inhibition (-30%) of basal thromboxane B2 formation. This result is consistent with the observed inhibitory effects of flavonoids on platelet aggregation. DHA treatment of platelets decreased the amount of a-tocopherol and increased the malondialdehyde (MDA) formation. The presence of epicatechin or catechin prevented the oxidative stress induced by DHA. Indeed, the platelet a-tocopherol level was significantly increased when compared to that of platelets treated by DHA alone, to reach the control level, whereas MDA was depressed to a level even lower than the control one. Our results indicate that catechin and especially epicatechin protect platelets from a peroxidative stress.
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References
- 1 Bick RL. Platelet function defects associated with hemorrhage or thrombosis. Med Clin North America 1994; 78: 577-560
- 2 Hamberg M, Svensson J, Samuelsson B. Thromboxanes: a new group of biologically active compounds derived from prostaglandin endoperoxides. Proc Natl Acad Sci USA 1975; 72: 2994-2998
- 3 Nugteren DH. Arachidonate lipoxygenase in blood platelets. Biochim Biophys Acta 1975; 380: 299-307
- 4 Bryant RW, Bailey JM. Altered lipoxygenase metabolism and decreased glutathione peroxidase activity in platelets from selenium-deficient rats. Biochem Biophys Res Commun 1980; 92: 268-276
- 5 Bang HO, Dyerberg J, Nielsen A. Plasma lipid and lipoprotein pattern in Greenlandic West coast Eskimos. Lancet 1971; 1: 1143-1146
- 6 Thomgren M, Gustafson A. Effects of 11-week increase in dietary eicosa-pentaenoic acid on bleeding time, lipids, and platelet aggregation. Lancet 1981; 2: 1190-1193
- 7 Gardner HW. Oxygen radical chemistry of polyunsaturated fatty acids. Free Rad Biol Med 1989; 7: 65-86
- 8 Iritani N, Fukuda E, Kitamura Y. Effect of com oil feeding on lipid peroxidation in rats. J Nutr 1980; 110: 924-930
- 9 Brown JE, Wahle KWJ. Effect of fish oil and vitamin E supplementation on lipid peroxidation and whole blood aggregation in man. Clin Chim Acta 1990; 193: 147-156
- 10 Harats D, Dabach Y, Ben-Naim M, Schwartz R, Berry EM, Stein O, Stein Y. Fish oil ingestion in smokers and nonsmokers enhances peroxidation of plasma lipoproteins. Atherosclerosis 1991; 90: 127-139
- 11 Halliwell B, Gutteridge JMC. Free radicals, ageing, and disease. In: Free Radicals in Biology and Medicine Halliwell B, Gutteridge JMC. eds. New York: Oxford: University Press; 1989: 416-508
- 12 Bors W, Heller W, Michel C, Saran M. Flavonoids as antioxidants: determination of radical scavenging, efficiencies. Methods Enzymol 1990; 186: 343-355
- 13 Hertog MGL, Hollman PCH, van de Putte B. Content of potentially anticar-cinogenic flavonoids of tea infusions, wines and fruit juices. J Agric Food Chem 1993; 41: 1242-1246
- 14 Fraga CG, Martino VS, Ferrago GE, Coussio JD, Boveris A. Flavonoids as antioxidants evaluated in vitro and in situ liver chemiluminescence. Biochem Pharmacol 1987; 36: 717-720
- 15 Ratty AK, Sunamoto J, Das P. Interaction of flavonoids with 1,1 -dipheny 1-2-picryhydrazyl free radical, liposomal membranes and soybean lipoxygenase 1. Biochem Pharmacol 1988; 37: 989-995
- 16 Lagarde M, Bryon PA, Guichardant M, Dechavanne M. A simple and efficient method for platelet isolation from their plasma. Thromb Res 1980; 17: 581-588
- 17 Lagarde M, Drouot B, Guichardant M, Dechavanne M. In vitro incorporation and metabolism of some icosaenoic acids in platelets. Effect on arach-idonic acid oxygenation. Biochim Biophys Acta 1985; 833: 52-58
- 18 Bom GVR. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 1962; 4: 927-929
- 19 Véricel E, Croset M, Sedivy P, Courpron Ph, Dechavanne M, Lagarde M. Platelets and Aging. I-Aggregation, Arachidonate metabolism and Antioxidant status. Thromb Res 1988; 49: 331-342
- 20 Therasse J, Lemonnier F. Determination of plasma lipoperoxides by high performance liquid chromatography. J Chromatogr 1987; 413: 237-241
- 21 Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158-169
- 22 Chaudière J, Gérard D. Dosage de l’activité glutathion peroxydase. In: Bio-logie des Lipides Chez l’Homme Douste-Blazy L, Mendy F. eds Editions Internationales; Paris: 1988: 275-289
- 23 Kromann N, Green A. Epidemiological studies in the Upernavik district, Greenland. Acta Med Scand 1980; 208: 401-406
- 24 Hirai A, Terano R, Saito H, Tamura Y, Yoshida S, Sajiki J, Kumagai A. Eicosapentaenoic acid and platelet function in Japanese. In: Nutritional Prevention of Cardiovascular Disease Goto Y, Homma Y. eds New York: Academic Press; 1984: 231-239
- 25 Goodnight SH, Harris WS, Connor WE, Illingworth DR. Polyunsaturated Fatty Acids, Hyperlipidemia, and Thrombosis. Arteriosclerosis 1982; 2: 87-113
- 26 Piche LA, Draper HH, Cole PD. Malondialdehyde excretion by subjects consuming cod liver oil vs a concentrate of n-3 fatty acids. Lipids 1988; 23: 370-371
- 27 Siess W, Roth P, Scherer B, Kurzmann I, Bohlig B, Weber PC. Platelet membrane fatty acids, platelet aggregation, and thromboxane formation during a mackerel diet. Lancet 1980; 1: 441-444
- 28 Bruna E, Petit E, Beljean-Leymarie M, Huynh S, Nouvelot A. Specific susceptibility of docosahexaenoic acid and eicosapentaenoic acid to peroxidation in aqueous solution. Lipids 1989; 24: 970-975
- 27 Blum U. Plants defend themselves chemicaly. In: Research for tomorrow, Yearbook of Agriculture U.S. Department of Agriculture Crowley JJ. ed Washington, USA: 1986: 139-142
- 30 Cazenave JP, Beretz A, Anton R. In: Flavonoids and Bioflavonoids. The effects of flavonoids on human platelet function Farkas L, Gabor M, Kallay F. eds Elsevier Science Publishers; Amsterdam: 1986: 373-380
- 31 Baumann J, Bruchhausen FV, Wurm G. Flavonoids and related compounds as inhibitors of arachidonic acid. Prostaglandins 1980; 20: 627-639
- 32 Corvazier E, Maclouf J. Interference of some flavonoids and non-steroidal anti-inflammatory drugs with oxidative metabolism of arachidonic acid by human platelets and neutrophils. Biochim Biophys Acta 1985; 835: 315-321
- 33 Pace-Asciak CR, Hahn S, Diamandis EP, Soleas G, Goldberg DM. The red wine phenolics trans-resveratrol and quercetin block human platelet aggregation and eicosanoid synthesis: Implications for protection against coronary heart disease. Clin Chim Acta 1995; 235: 207-219
- 34 Terao J, Piskula M, Yao Q. Protective effect of epicatechin, epicatechin gallate, and quercetin on lipid peroxidation in phospholipid bilayers. Arch Biochem Biophys 1994; 308: 278-284
- 35 Flohe L. The selenoprotein glutathione peroxidase. In: Glutathione, Chemical, Biochemical and Medical Aspects Dolphin D, Poulson R, Avamoric O. eds New York: Wiley; 1989: 644-731
- 36 Lands WEM, Kulmacz RJ. The regulation of the biosynthesis of prostaglandins and leukotrienes. Prog Lipid Res 1986; 25: 105-109
- 37 Lagarde M. Metabolism of fatty acids by platelets and the functions of various metabolites in mediating platelet function. Prog Lipid Res 1988; 27: 135-152
- 38 Joulain C, Prigent AF, Nemoz G, Lagarde M. Increased glutathione peroxidase activity in human blood mononuclear cells upon in vitro incubation with n-3 fatty acids. Biochem Pharmacol 1994; 47: 1315-1323
- 39 Bellisola G, Galassini S, Moschini G, Poli G, Perona G, Guidi GC. Selenium and glutathione peroxidase variations induced by polyunsaturated fatty acids oral supplementation in humans. Clin Chim Acta 1992; 205: 75-85
- 40 Vericel E, Rey C, Calzada C, Haond P, Chapuy PH, Lagarde M. Age-related changes in arachidonic acid peroxidation and glutathione-peroxidase activity in human platelets. Prostaglandins 1992; 43: 75-85
- 41 Hertog MGL, Feskens EJM, Hollman PCH, Katan MB, Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet 1993; 342 1007-1011
- 42 Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Rad Res 1995; 22: 375-383
- 43 Frankel EN, Waterhouse AL, Teissedre PL. Principal phenolic phytochemicals in selected California wines and their antioxidant activity in inhibiting oxidation of human low-density lipoproteins. J Agric Food Chem 1995; 43: 890-894
- 44 Renaud S, De Lorgeril M. Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet 1992; 339: 1523-1526
- 45 St Leger AS, Cochrane AL, Moore F. Factors associated with cardiac mortality in developed countries with particular reference to the consumption of wine. Lancet 1979; i: 1017-1020