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Thromb Haemost 1996; 75(06): 945-949
DOI: 10.1055/s-0038-1650399
Original Article
Schattauer GmbH Stuttgart

N-3 Fatty Acid-induced Lipid Peroxidation in Human Platelets is Prevented by Catechins

Anne Polette
The Institut National de la Santé et de la Recherche Médicale U.352, Chimie Biologique INSA-Lyon, Villeurbanne, France
,
Dominique Lemaitre
The Institut National de la Santé et de la Recherche Médicale U.352, Chimie Biologique INSA-Lyon, Villeurbanne, France
,
Michel Lagarde
The Institut National de la Santé et de la Recherche Médicale U.352, Chimie Biologique INSA-Lyon, Villeurbanne, France
,
Evelyne Véricel
The Institut National de la Santé et de la Recherche Médicale U.352, Chimie Biologique INSA-Lyon, Villeurbanne, France
› Author Affiliations
Further Information

Publication History

Received 21 November 1995

Accepted after resubmission 06 March 1996

Publication Date:
11 July 2018 (online)

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Summary

The effects of catechins were studied in both unstimulated platelets and platelets submitted to a mild oxidative stress induced by docosa-hexaenoic acid (DHA). Human unstimulated platelets were incubated in the presence of catechin or epicatechin (1, 10 or 100 μM) for 2 h at 37° C. Treatment by 1 μM epicatechin was sufficient to induce a significant inhibition (-30%) of basal thromboxane B2 formation. This result is consistent with the observed inhibitory effects of flavonoids on platelet aggregation. DHA treatment of platelets decreased the amount of a-tocopherol and increased the malondialdehyde (MDA) formation. The presence of epicatechin or catechin prevented the oxidative stress induced by DHA. Indeed, the platelet a-tocopherol level was significantly increased when compared to that of platelets treated by DHA alone, to reach the control level, whereas MDA was depressed to a level even lower than the control one. Our results indicate that catechin and especially epicatechin protect platelets from a peroxidative stress.

 

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