Expression of Plasminogen Activator Inhibitor 2 in the Adult and Embryonic Mouse Tissues

Yoichi Kawata; Jun Mimuro; Munekiyo Kaneko; Kazuyuki Shimada; Yoichi Sakata

doi:10.1055/s-0038-1650624

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1996; 76(04): 569-576
DOI: 10.1055/s-0038-1650624

Original Article

Schattauer GmbH Stuttgart

Expression of Plasminogen Activator Inhibitor 2 in the Adult and Embryonic Mouse Tissues

Yoichi Kawata

¹The Institute of Hematology, Jichi Medical School, Japan

²The Department of Cardiology, Jichi Medical School, Japan

,

Jun Mimuro

¹The Institute of Hematology, Jichi Medical School, Japan

,

Munekiyo Kaneko

¹The Institute of Hematology, Jichi Medical School, Japan

,

Kazuyuki Shimada

²The Department of Cardiology, Jichi Medical School, Japan

,

Yoichi Sakata

¹The Institute of Hematology, Jichi Medical School, Japan

› Author Affiliations

Abstract

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Summary

We have studied the expression of plasminogen activator inhibitor 2 (PAI-2) in the adult mouse and during embryonic development using immunohistochemistry and the polymerase-chain reaction. Mouse PAI-2 mRNA was mainly expressed in the skin, bone-marrow, spleen, lung, thymus, and urinary bladder. Immunohistochemical studies suggested that PAI-2 was synthesized in macrophages and epithelial cells such as skin epithelial cells, transitional cells of the urinary bladder, and mesothelial cell of peritoneum and pleura. PAI-2 mRNA and antigen expression was observed only in the skin of 15 day-old mouse embryos. These data suggest that PAI-2 may play a role in protecting the mouse embryo from the protease attack in the amniotic fluid and also in maintaining and/or protecting the structure of various surfaces in the mouse.

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References

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