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Thromb Haemost 1996; 76(06): 0977-0982
DOI: 10.1055/s-0038-1650695
Original Article
Schattauer GmbH Stuttgart

European Concerted Action on Anticoagulation (ECAA) - The Multicentre Calibration of Rabbit and Human ECAA Reference Thromboplastins

L Poller
1   The Central Facility, ECAA, Department of Pathological Sciences, The University of Manchester, UK
,
T W Barrowcliffe
2   Division of Haematology, National Institute for Biological Standards and Control, UK
,
A M H P van den Besselaar
3   Haemostasis and Thrombosis Research Centre, Leiden University Hospital, The Netherlands
,
J Jespersen
4   Department of Clinical Biochemistry, Centralsygehuset, Esbjerg, Denmark
,
A Tripodi
5   A Bianchi Bonomi Hemophilia and Thrombosis Centre, University & IRCCS Maggiore Hospital, Milan, Italy
,
D Houghton
1   The Central Facility, ECAA, Department of Pathological Sciences, The University of Manchester, UK
,
Prepared on behalf of the ECAA by the above steering committee › Author Affiliations
Further Information

Publication History

Received 29 May 1996

Accepted after revision 09 August 1996

Publication Date:
11 July 2018 (online)

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Summary

Two candidate ECAA reference preparations have been calibrated in a multicentre exercise at 14 representative national laboratories to provide reference thromboplastin for a large field study in 16 European countries. Two preparations were required because of the established differences in ISI arising from the two main routes of calibration via rabbit and human IRP. To be comparable with working reagents in everyday use a human plain recombinant reagent of low ISI and a rabbit plain preparation of moderately high ISI were selected. A precise calibration of the two candidate preparations has been achieved with the manual PT technique and an ISI of 0.95 (SE 0.0078) for the human reagent and 1.67 (SE 0.0322) for the rabbit reagent.

 

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