Thromb Haemost 1996; 76(06): 1086-1089
DOI: 10.1055/s-0038-1650710
Original Article
Schattauer GmbH Stuttgart

Soluble P-selectin as Parameter for Platelet Activation during Storage

E H Kostelijk
The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam
,
R Fijnheer
1   The Department of Haematology, University Hospital Utrecht, Utrecht, The Netherlands
,
H K Nieuwenhuis
1   The Department of Haematology, University Hospital Utrecht, Utrecht, The Netherlands
,
C W N Gouwerok
The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam
,
D de Korte
The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 18. März 1996

Accepted after revision 20. August 1996

Publikationsdatum:
11. Juli 2018 (online)

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Summary

Platelet concentrates stored at room temperature deteriorate. The so-called storage lesion is characterised by morphological changes and a loss of functionality. To find an assay for early platelet activation in platelet concentrates the morphological score, β-TG release and P-selectin expression were determined, and compared with the amount of soluble P-selectin. An ELISA was used to quantify soluble P-selectin in the storage medium. We found a significant correlation between the amount of soluble P-selectin and the percentage of P-selectin positive platelets (flow-cytometric analysis) (r = 0.7449; p <0.0001) or the amount of β-TG release (r = 0.6837; p<0.0001). The morphological score also correlated significantly (negative) with the amount of soluble P-selectin (r = -0.7669; p = 0.0002). From day 0 till day 8, the amount of soluble P-selectin increased constantly from 219 ± 49.2ng/ml to 556 ± 102.3 ng/ml. The detection of soluble P-selectin can be used to quantify activation of platelets during storage. The immuno-assay for soluble P-selectin is more sensitive than flow-cytometric analysis of the percentage of P-selectin-positive cells and allows earlier detection of platelet activation.