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Thromb Haemost 1989; 62(03): 885-891
DOI: 10.1055/s-0038-1651022
Original Article
Schattauer GmbH Stuttgart

Protein C Inhibitor from Human Plasma: Characterization of Native and Cleaved Inhibitor and Demonstration of Inhibitor Complexes with Plasma Kallikrein

Martin Laurell
The Department of Clinical Chemistry, Malmö General Hospital Malmö, Sweden
,
Johan Stenflo
The Department of Clinical Chemistry, Malmö General Hospital Malmö, Sweden
› Author Affiliations
Further Information

Publication History

Received 17 March 1989

Accepted after revision 20 July 1989

Publication Date:
30 June 2018 (online)

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Summary

Protein C inhibitor (PCI) was purified from human plasma using immunoaffinity chromatography and heparin Sepharose chromatography, a method that allowed the purification of active and inactive inhibitor. PCI purified from outdated plasma was inactive and either in complex with plasma kallikrein or proteo-lytically degraded. Sequence analysis of cleaved PCI and of complexes between PCI and activated protein C or urokinase identified the previously recognized inhibitor cleavage site Arg354-Ser355. Two additional cleavage sites were observed in the modified inhibitor i.e. Arg357-Leu358 and Arg362-Leu363 which probably represent secondary cleavage of the inhibitor. Furthermore the sequence analysis of the inhibitor, whether purified from fresh or outdated plasma, revealed that it was microheterogeneous in the NH2-terminus as a result of cleavage by a trypsin like enzyme(s).

Keywords

Protein C - Protein C inhibitor - Plasma kallikrein - Urokinase - Heparin
 

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