Thrombin Stimulated Platelet Accumulation on Protein Coated Glass Capillaries: Role of Adhesive Platelet α-Granule Proteins

Juliette N Mulvihill; J Andrew Davies; Florence Toti; Jean-Marie Freyssinet; Jean-Pierre Cazenave

doi:10.1055/s-0038-1651040

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1989; 62(03): 989-995
DOI: 10.1055/s-0038-1651040

Original Article

Schattauer GmbH Stuttgart

Thrombin Stimulated Platelet Accumulation on Protein Coated Glass Capillaries: Role of Adhesive Platelet α-Granule Proteins

Juliette N Mulvihill

INSERM U.311. Biologie et Pharmacologie des Interactions du Sang avec les Vaisseaux et les Biomatériaux, Centre Régional de Transfusion Sanguine, Strasbourg, France

,

J Andrew Davies

^*University Department of Medicine, The General Infirmary, Leeds, United Kingdom

,

Florence Toti

INSERM U.311. Biologie et Pharmacologie des Interactions du Sang avec les Vaisseaux et les Biomatériaux, Centre Régional de Transfusion Sanguine, Strasbourg, France

,

Jean-Marie Freyssinet

INSERM U.311. Biologie et Pharmacologie des Interactions du Sang avec les Vaisseaux et les Biomatériaux, Centre Régional de Transfusion Sanguine, Strasbourg, France

,

Jean-Pierre Cazenave

INSERM U.311. Biologie et Pharmacologie des Interactions du Sang avec les Vaisseaux et les Biomatériaux, Centre Régional de Transfusion Sanguine, Strasbourg, France

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Abstract

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Summary

The generation of trace amounts of thrombin at artificial surfaces in contact with blood is likely to be a contributing factor in thrombosis on biomaterials. Using an in vitro capillary perfusion system, platelet accumulation on glass surfaces, uncoated or precoated with purified bovine collagen or human plasma proteins, was determined in the presence or absence of preadsorbed purified human thrombin. Static adsorption for 15 min at 22° C from solutions of thrombin 100 NIH units (33 μg)/ml gave surface concentrations in the range 0.019-0.101 μg/cm². Protein coated capillaries, thrombin treated or untreated, were perfused for 2 min at 37° C with suspensions of washed ¹¹¹In-labeled human platelets in Tyrode's-albumin buffer containing 40% washed red blood cells, under conditions of controlled, non pulsatile laminar flow (50 s⁻¹ or 2,000 s⁻¹). Platelet accumulation was increased in the presence of surface adsorbed thrombin on uncoated and albumin or fibrinogen coated glass but little affected on fibronectin or collagen coated glass. On von Willebrand factor (vWF) coated glass, thrombin enhancement was observed only at high shear forces. In experiments using antibodies against human platelet α-granule proteins, thrombin stimulated platelet deposition in uncoated glass capillaries was inhibited at 2,000 s⁻¹ by anti-vWF and to a lesser extent by anti-fibrinogen but not by antithrombospondin antibodies.

Keywords

Platelet accumulation - Capillary perfusion - Thrombin - Adhesive α-granule protein - Biomaterials

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Referenzen

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