Inhibition of Platelet Thromboxane Formation and Phosphoinositides Breakdown by Osthole from Angelica pubescens

Feng-Nien Ko; Tian-Shung Wu; Meei-Jen Liou; Tur-Fu Huang; Che-Ming Teng

doi:10.1055/s-0038-1651041

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1989; 62(03): 996-999
DOI: 10.1055/s-0038-1651041

Original Article

Schattauer GmbH Stuttgart

Inhibition of Platelet Thromboxane Formation and Phosphoinositides Breakdown by Osthole from Angelica pubescens

Feng-Nien Ko

The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, Taiwan

,

Tian-Shung Wu

^*The Department of Applied Chemistry, Providence College of Arts and Science, Shalu, Taichung-Hsien, Taiwan

,

Meei-Jen Liou

^*The Department of Applied Chemistry, Providence College of Arts and Science, Shalu, Taichung-Hsien, Taiwan

,

Tur-Fu Huang

The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, Taiwan

,

Che-Ming Teng

The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, Taiwan

› Author Affiliations

Abstract

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Summary

Osthole, isolated from Chinese herb Angelica pubescens, inhibited platelet aggregation and ATP release induced by ADP, arachidonic acid, PAF, collagen, ionophore A23187 and thrombin in washed rabbit platelets. It showed a weak activity in platelet-rich plasma. Osthole inhibited the thromboxane B₂ formation caused by arachidonic acid, collagen, ionophore A23187 and thrombin in washed platelets, and also the thromboxane B₂ formation caused by the incubation of lysed platelet homogenate with arachidonic acid. The generation of inositol phosphates in washed platelets caused by collagen, PAF and thrombin was suppressed by osthole. These data indicate that the inhibitory effect of osthole on platelet aggregation and release reaction was due to the inhibition of thromboxane formation and phosphoinositides breakdown.

Keywords

Platelet inhibition - Thromboxane formation - Phosphoinositides breakdown - Osthole - Angelica pubescens

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References

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