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Thromb Haemost 1987; 57(01): 001-010
DOI: 10.1055/s-0038-1651051
Original Articles
Schattauer GmbH Stuttgart

Abnormal Aggregation Accompanies Abnormal Platelet Ca2+ Handling in Arterial Thrombosis

Nabila M Shanbaky
The Department of Medicine Medical School of the University of Miami, Miami, FL, USA
,
Yeon Ahn
*   The Department of Pharmacology and the Center for Blood Diseases, Miami, FL, USA
,
Wenche Jy
The Department of Medicine Medical School of the University of Miami, Miami, FL, USA
,
William Harrington
*   The Department of Pharmacology and the Center for Blood Diseases, Miami, FL, USA
,
Luis Fernandez
*   The Department of Pharmacology and the Center for Blood Diseases, Miami, FL, USA
,
Duncan H Haynes
The Department of Medicine Medical School of the University of Miami, Miami, FL, USA
› Institutsangaben
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Publikationsverlauf

Received 01. April 1986

Accepted after revision 29. September 1986

Publikationsdatum:
06. Juli 2018 (online)

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Summary

The resting levels of cytoplasmic Ca2+ (measured by Quin 2 fluorescence) and dense tubular Ca2+ (measured by chlorotetracycline, CTC, fluorescence) are shown to be higher in platelets from patients with arterial thrombosis than from normal donors. Turbidmetric studies of aggregation of diluted platelet-rich plasma (PRP) at 135 μM Ca2+ showed increased rates of aggregation for patients relative to normal controls. For ADP-stimulated aggregation, increased maximal rates (Vmax) and decreased doses for half-maximal rates were observed. With collagen-stimulated aggregation, patient samples showed only decreased ED50 values relative to normal controls. The changes in these values are linearly correlated with the elevation of resting dense tubular Ca2+ level determined by the calcium-CTC test carried out at 2 mM external Ca2+. For ADP-stimulated aggregation this relationship can be mimicked by pre-incubating normal platelets with subcritical concentrations of the Ca2+ ionophore A23187.

These results suggest that elevated cytoplasmic and dense tubular Ca2+ in the “resting state” is a major factor in arterial thrombosis, rendering the platelet more sensitive to the stimulation by physiologic agents.

Keywords

Platelet - Arterial thrombosis - Platelet hyperactivation - Platelet aggregation - Ca2+ uptake and release - Dense tubules - Chlorotetracycline - Quin 2
 

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