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Thromb Haemost 1987; 57(01): 017-019
DOI: 10.1055/s-0038-1651053
Original Articles
Schattauer GmbH Stuttgart

Substrate Recognition by Vitamin K-Dependent Carboxylase

Magda M W Ulrich
The Department of Biochemistry, University of Limburg, Maastricht, The Netherlands
,
Berry A M Soute
The Department of Biochemistry, University of Limburg, Maastricht, The Netherlands
,
L Johan M van Haarlem
The Department of Biochemistry, University of Limburg, Maastricht, The Netherlands
,
Cees Vermeer
The Department of Biochemistry, University of Limburg, Maastricht, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 28 July 1986

Accepted after revision 01 October 1986

Publication Date:
06 July 2018 (online)

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Summary

Decarboxylated osteocalcins were prepared and purified from bovine, chicken, human and monkey bones and assayed for their ability to serve as a substrate for vitamin K-dependent carboxylase from bovine liver. Substantial differences were observed, especially between bovine and monkey d-osteocalcin. Since these substrates differ only in their amino acid residues 3 and 4, it seems that these residues play a role in the recognition of a substrate by hepatic carboxylase.

Keywords

Vitamin K - Carboxylase - Osteocalcin - Gammacarboxy-glutamic acid
 

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