Substrate Recognition by Vitamin K-Dependent Carboxylase

Summary

Decarboxylated osteocalcins were prepared and purified from bovine, chicken, human and monkey bones and assayed for their ability to serve as a substrate for vitamin K-dependent carboxylase from bovine liver. Substantial differences were observed, especially between bovine and monkey d-osteocalcin. Since these substrates differ only in their amino acid residues 3 and 4, it seems that these residues play a role in the recognition of a substrate by hepatic carboxylase.

Keywords

Vitamin K - Carboxylase - Osteocalcin - Gammacarboxy-glutamic acid

• References

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