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Thromb Haemost 1987; 57(01): 044-048
DOI: 10.1055/s-0038-1651059
Original Articles
Schattauer GmbH Stuttgart

Multicenter Comparison of Five Functional and Two Immunological Assays for Protein C

P M Mannucci
1   The A. Bianchi Bonomi Hemophilia and Thrombosis Center and the Institute of Internal Medicine, University of Milano, Italy
,
C Boyer
2   The Laboratoire Central d’Hématologie and INSERM U.143, Hôpital Bicêtre, Le Kremlin-Bicêtre, France
,
A Tripodi
1   The A. Bianchi Bonomi Hemophilia and Thrombosis Center and the Institute of Internal Medicine, University of Milano, Italy
,
S Viganò-D'Angelo
3   The Thrombosis/Hematology Research Program Oklahoma Medical Research Foundation, Oklahoma City, USA
,
M Wolf
2   The Laboratoire Central d’Hématologie and INSERM U.143, Hôpital Bicêtre, Le Kremlin-Bicêtre, France
,
C Valsecchi
1   The A. Bianchi Bonomi Hemophilia and Thrombosis Center and the Institute of Internal Medicine, University of Milano, Italy
,
A D'Angelo
1   The A. Bianchi Bonomi Hemophilia and Thrombosis Center and the Institute of Internal Medicine, University of Milano, Italy
,
D Meyer
2   The Laboratoire Central d’Hématologie and INSERM U.143, Hôpital Bicêtre, Le Kremlin-Bicêtre, France
,
M J Larrieu
2   The Laboratoire Central d’Hématologie and INSERM U.143, Hôpital Bicêtre, Le Kremlin-Bicêtre, France
› Author Affiliations
Further Information

Publication History

Received 02 June 1986

Accepted after revision 27 October 1986

Publication Date:
06 July 2018 (online)

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Summary

Five functional assays and two immunoassays for protein C (PC) were evaluated in parallel for the same plasma samples collected from healthy subjects, patients with congenital and acquired PC deficiencies or patients with conditions associated with high PC levels. For 7 patients starting warfarin therapy and for 15 patients during stabilized warfarin therapy, there were significant between-assay differences. For these groups immunoassays gave higher values than most functional assays and the latter also gave varied results, probably depending on their respective capacity for recognizing acarboxylated PC. On the other hand, there were no significant between-assay differences nor discrepancies between PC activity and antigen levels for healthy subjects (n = 39), patients with congenital PC deficiency (n = 10), myocardial infarction (n = 25), chronic liver disease (n = 19), disseminated intravascular coagulation (n = 35), in the post-operative period (n = 20) or in women taking oral contraceptives (n = 20). This comparison of PC assays indicates that PC levels measured by different functional or immunological assays are very close in the majority of clinical conditions, but not for patients on oral anticoagulants.

Keywords

Protein C - Protein C assays
 

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