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Thromb Haemost 1987; 57(03): 278-282
DOI: 10.1055/s-0038-1651116
Original Article
Schattauer GmbH Stuttgart

Characterisation of a Monoclonal Antibody to von Willebrand Factor as a Potent Inhibitor of Ristocetin-Mediated Platelet Interaction and Platelet Adhesion

Sylvie Jorieux
The Laboratoire d’Hémostase du Centre Régional de Transfusion Sanguine de Lille, France
,
Christophe de Romeuf
The Laboratoire d’Hémostase du Centre Régional de Transfusion Sanguine de Lille, France
,
Bruno Samor
The Laboratoire d’Hémostase du Centre Régional de Transfusion Sanguine de Lille, France
,
Maurice Goudemand
The Laboratoire d’Hémostase du Centre Régional de Transfusion Sanguine de Lille, France
,
Claudine Mazurier
The Laboratoire d’Hémostase du Centre Régional de Transfusion Sanguine de Lille, France
› Author Affiliations
Further Information

Publication History

Received 28 July 1986

Accepted after revision 16 February 1987

Publication Date:
06 July 2018 (online)

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Summary

We studied a murine monoclonal antibody (211 A6) to von Willebrand factor (vWF) with a view to investigating structure-relationship. of plasma vWF. The specificity of this antibody has been substantiated by ELISA tests and indirect immunofluorescence. It reacts with purified vWF, normal plasma but not with plasma or platelets from a severe von Willebrand’s disease patient. Monoclonal antibody 211 A6 is a potent inhibitor of ristocetin-induced platelet aggregation. The 125I-FVIII/vWF binding to platelets in presence of ristocetin is totally inhibited by low 211 A6 concentrations. Thrombin-induced binding of vWF to platelets is not affected by 211 A6. The ability of this antibody to inhibit platelet adhesion to subendothelium and to collagen was investigated with a perfusion model. The complete inhibition of platelet adhesion by 211 A6 questions the similarity or the interrelationship in vWF domains involved in ristocetin-induced platelet functions and platelet adhesion.

Keywords

Monoclonal antibody - von Willebrand factor - Ristocetin cofactor - Platelet adhesion
 

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