Transformation of Autoprothrombin III to Autoprothrombin C in Sodium Citrate Solution

R Kipfer; W. H Seegers

doi:10.1055/s-0038-1651197

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1968; 19(01/02): 204-212
DOI: 10.1055/s-0038-1651197

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Transformation of Autoprothrombin III to Autoprothrombin C in Sodium Citrate Solution^[*]

R Kipfer

¹Department of Physiology and Pharmacology, Wayne State University, School of Medicine, Detroit, Michigan, U.S.A.

,

W. H Seegers

¹Department of Physiology and Pharmacology, Wayne State University, School of Medicine, Detroit, Michigan, U.S.A.

› Author Affiliations

Abstract

Summary

All reactions studied occurred in 25% sodium citrate solution. The conversion of prethrombin to thrombin with autoprothrombin C was retarded by 3,4,4’-triaminodi-phenyl sulfone. The compound functioned as a competitive inhibitor. Purified autoprothrombin III converted to autoprothrombin C more rapidly when autoprothrombin C was added at zero time. Soybean trypsin inhibitor, which neutralizes autopro-thrombin C activity, blocked the conversion of autoprothrombin III to autoprothrom-bin C, and 3,4,4’-triaminodiphenylsulf one inhibited the development of autoprothrombin C activity. The activation of prothrombin in 25% sodium citrate solution consists of three main events; namely, 1. the dissociation of prothrombin into subunits, 2. the formation of autoprothrombin C, and 3. the formation of thrombin.

Full Text

References

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Transformation of Autoprothrombin III to Autoprothrombin C in Sodium Citrate Solution[*]

Transformation of Autoprothrombin III to Autoprothrombin C in Sodium Citrate Solution^[*]