Download PDF
Thromb Haemost 1968; 20(03/04): 469-476
DOI: 10.1055/s-0038-1651290
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

The Effects of Infusing Thrombin and Its Acetylated Derivative

II. Observations on Intravascular Hemolysis with Pathologic Correlations
J. M Bennett   *
1   Departments of Medicine and Pathology, Beth Israel Hospital and Harvard Medical School, Boston, Mass.
,
Dinah Yu
1   Departments of Medicine and Pathology, Beth Israel Hospital and Harvard Medical School, Boston, Mass.
,
J Suyemoto
1   Departments of Medicine and Pathology, Beth Israel Hospital and Harvard Medical School, Boston, Mass.
,
L Pechet   **
1   Departments of Medicine and Pathology, Beth Israel Hospital and Harvard Medical School, Boston, Mass.
› Author AffiliationsSupported in part by research grants HE-00656, HE-06316, NIH, USPHS.
Further Information

Publication History

Publication Date:
27 June 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary and Conclusions

In conjunction with experiments designed to test the effects of infusing acetylated thrombin (Thrombin E) and Clotting thrombin (Thrombin C) into dogs, the occurrence of hemolysis was noted. Serial observations on its magnitude, its relationship to thrombin-induced defibrination, and the formation of thrombi were made. The findings suggest a unique role for thrombin in inducing “in vivo” intravascular hemolysis in short term experiments, possibly via an indirect action on red blood cells.

* Present address: Hematology Service, Clinical Pathology Department, National Institutes of Health, Bethesda, Md. 20014.


** Department of Pathology, and Medicine, Univ. Colorado School of Medicine, Veterans Administration Hospital, Denver, Colorado.


 

  • References

  • 1 Pecket L, Engel A.M, Goldstein C, Glaser B. The Effects of Infusing Thrombin and Its Acetylated Derivative: I: Studies of Coagulation and Fibrinolysis. Thrombos. Diath. haemorrh. (Stuttg.) 1968; 20: 190
    PubMedSearch in Google Scholar
  • 2 Jenkins D.E, Lyde N.L, Hartmann R.C. Studies of bovine thrombin enhancement of acid hemolysis reaction of paroxysmal nocturnal hemoglobinuria. J. clin. Invest 1964; 43: 1275
    PubMedSearch in Google Scholar
  • 3 Miller K.A, van Vunakis H. The Effect of Diisopropylflourophosphate on the Proteinase and Esterase Activities of Thrombin and on Prothrombin and its Activators. J. biol. Chem 1956; 225: 227
    PubMedSearch in Google Scholar
  • 4 Goldstein R, LeBolloc’h A, Alexander B, Zonderman E. Preparation and properties of prothrombin. J. biol. Chem 1959; 234: 2857
    PubMedSearch in Google Scholar
  • 5 Seegers W.H, McGlaughry R.I, Fahey J.L. Some properties of purified prothrombin and its activation with sodium citrate. Blood 1950; 5: 421
    PubMedSearch in Google Scholar
  • 6 Crosby Jr. W.H, Murin J.I, Furth F.W. Standardizing a method for clinical hemo-globinometry. U. S. Armed Forces Med. J 1954; 51: 693
    PubMedSearch in Google Scholar
  • 7 Young L.E, Izzo M.J, Altman K.J, Swisher S.N. Studies on Spontaneous In Vitro Autohemolysis in Hemolytic Disorders. Blood 1956; 11: 977
    PubMedSearch in Google Scholar
  • 8 Warner E.D, Brinkhous K.M, Seegers W.H, Smith H.P. Further Experience with the use of Thrombin as a Hemostatic agent. Proc. Soc. exper. Biol. (N. Y.) 1939; 41: 655
    CrossrefPubMedSearch in Google Scholar
  • 9 Quick A.J, Hussey C.V, Harris J, Peters K. Occult intravascular clotting by means of thrombin. Amer. J. Physiol 1959; 197: 791
    PubMedSearch in Google Scholar
  • 10 Hardaway R.M, Watson H.E, Weiss F.H. Alterations in blood coagulation mechanism after intra-aortic injection of thrombin. Arch. Surg 1960; 81: 983
    CrossrefPubMedSearch in Google Scholar
  • 11 Brain M.C, Dacie J.V.D, Hourihane D. O. B. Microangiopathic Haemolytic Anemia: The Possible Role of Vascular Lesions in Pathogenesis. Brit. J. Haemat 1962; 8: 358
    CrossrefPubMedSearch in Google Scholar
  • 12 Pritchard J.A, Weisman Jr. R, Ratnoff O.D, Vosburgh G.J. Intravascular Hemolysis. Thrombocytopenia, and other hematologic abnormalities associated with severe toxemia of pregnancy. New Engl. J. Med 1954; 250: 89
    CrossrefPubMedSearch in Google Scholar
  • 13 Blum S.F, Gardner F.H. Paroxysmal Nocturnal Hemoglobinuria: Mechanism of the Enhancement of Hemolysis by Bovine Thrombin. Blood 1967; 30: 352
    PubMedSearch in Google Scholar
  • 14 Storti E, Vaccari F, Baldini E. Studies on Relationship between Anticoagulants and Hemolysis. Acta haemat. (Basel) 1956; 15: 106
    CrossrefPubMedSearch in Google Scholar
  • 15 Pas A.T, Monroe R.W. The Treatment of Autoimmune Hemolytic Anemia with Heparin. Amer. J. med. Sci 1966; 251: 101
    PubMedSearch in Google Scholar
  • 16 Brain M.C, Hourihane D.O. B. The Occurrence of Haemolysis in Experimentally Produced Vascular Disease. Brit. J. Haemat 1967; 13: 135
    CrossrefPubMedSearch in Google Scholar
  • 17 Regoeczi E, Rubenberg M.L, Brain M.C. Intravascular Hemolysis and Disseminated Intravascular Coagulation. Lancet 1967; I: 601
    PubMedSearch in Google Scholar
  • 18 Ratnoff O.B, Menzie C. A New Method for the Determination of Fibrinogen in Small Samples of Plasma. J. Lab. clin. Med 1951; 37: 316
    PubMedSearch in Google Scholar
  • 19 Goodwin Y.F. Estimation of Plasma Fibrinogen. Amer. J. clin. Path 1961; 35: 227
    PubMedSearch in Google Scholar