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Thromb Haemost 1969; 22(03): 577-583
DOI: 10.1055/s-0038-1651394
DOI: 10.1055/s-0038-1651394
Vorläufige Mitteilungen — Preliminary Reports — Communications Préliminaires
Purification of the Antihemophilic Factor by Gel Filtration on Agarose
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Publikationsverlauf
Publikationsdatum:
10. Juni 2018 (online)
Summary
Factor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.
The purified factor VIII was active in vivo as well as in vitro.
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References
- 1 Lorand L, Laki K. A simple method for purifying an activator of prothrombin. Biochim. biophys. Acta (Amst.) 13: 448 1954;
- 2 Loeb J. Séparation du fibrinogène et du facteur antihémophilique A.I. A l’aide de kaolin. Path, et Biol. 7: 2449 1959;
- 3 Soulier J. P. Séparation du fibrinogène et du facteur anti-hémophilique A.II. A l’aide de la bentonite. Path, et Biol. 7: 2451 1959;
- 4 Blombäck B, Blombäck M, Struwe I. Studies on blood clotting: factor VIII. Ark. for Kemi 15: 223 1961/1962
- 5 van Creveld S, Pascha C. N, Veder H. A. The separation of A.H.F. from fibrinogen. Thrombos. Diathes. haemorrh. (Stuttg.) 15: 538 1966;
- 6 Veder H. A. Further Purification of the Antihaemophilic Factor (A.H.F.). Thrombos. Diathes. haemorrh. (Stuttg.) 16: 738 1966;
- 7 Wagner R. H, Lester W. D. M, Smith M, Brinkhous K. M. Purification of antihaemophilic Factor (Factor VIII) by amino acid precipitation. Thrombos. Diathes. haemorrh. (Stuttg.) 11: 64 1964;
- 8 Wieme R. J. Studies on agar gel electrophoresis. Arscia uitgaven N.V.Brussel: 201 (1959).
- 9 Hershgold E. J, Pool J. G, Pappenhagen A. R. The potent antihemophilic globulin concentrate derived from a cold insoluble fraction of human plasma : characterisation and further data on preparation and clinical trial. J. Lab. clin. Med. 67: 23 1966;
- 10 Ratnoff O. D, Kass L, Lang P. D. Rapid purification of human antihaemophilic factor (factor VIII). J. Lab. clin. Med. 72: 1007 1968;
- 11 Veltkamp J. J, Orion E. F, Loeliger E. A. Detection of the Carrier State in Hereditary Coagulation Disorders. Thrombos. Diathes. haemorrh. (Stuttg.) 19: 279 1968;
- 12 Poison F. A. Fractination of protein mixtures on columns of granulated agar. Biochim. bio-phys. Acta (Amst.) 50: 565 1961;
- 13 Pool J. G, Shannon A. E. Production of high-potency concentrates of antihemophilic globulin in closed bagsystem : Assay in vitro and in vivo. New Engl. J. Med. 273: 1443 1965;
- 14 Wagner A. Über den diagnostischen Wert der Eiweiß-gebundenen Hexosen. Klin. Wschr. 23: 1187 1960;
- 15 Elson L. A, Morgan W. T. J. A colormetric method for the determination of glucosamine and chondrosamine. Biochem. J. 27: 1824 1933;
- 16 Radhakrishnamurthy B, Berenson G. S. Differential determination of glucosamine and galac to samine. Clin. chim. Acta 10: 562 1964;
- 17 Böhm P, Dauber S, Baumeister L. Über Neuraminsäure, ihr Vorkommen und ihre Bedeutung im Serum. Klin. Wschr. 13: 289 1954;
- 18 Kuntz E, Arnold G. Die klinische Aktivitäts-Beurteilung der Lungentuberkulose. S.58. Georg Thieme; Stuttgart: 1963
- 19 Dische Z. A new specific color reaction of hexuronic acids. J. biol. Chem. 167: 189 1947;