Download PDF
Thromb Haemost 1969; 22(03): 577-583
DOI: 10.1055/s-0038-1651394
Vorläufige Mitteilungen — Preliminary Reports — Communications Préliminaires
Schattauer GmbH

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

M.M.P Paulssen
1   Department of Clinical Biochemistry, St. Clara Hospital, Rotterdam, Netherlands
,
A.C.M.G.B Wouterlood
1   Department of Clinical Biochemistry, St. Clara Hospital, Rotterdam, Netherlands
,
H.L.M.A Scheffers
1   Department of Clinical Biochemistry, St. Clara Hospital, Rotterdam, Netherlands
› Author Affiliations
Further Information

Publication History

Publication Date:
10 June 2018 (online)

Also available at
    Permissions and Reprints

Summary

Factor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.

The purified factor VIII was active in vivo as well as in vitro.

 

  • References

  • 1 Lorand L, Laki K. A simple method for purifying an activator of prothrombin. Biochim. biophys. Acta (Amst.) 13: 448 1954;
    CrossrefPubMedSearch in Google Scholar
  • 2 Loeb J. Séparation du fibrinogène et du facteur antihémophilique A.I. A l’aide de kaolin. Path, et Biol. 7: 2449 1959;
    PubMedSearch in Google Scholar
  • 3 Soulier J. P. Séparation du fibrinogène et du facteur anti-hémophilique A.II. A l’aide de la bentonite. Path, et Biol. 7: 2451 1959;
    PubMedSearch in Google Scholar
  • 4 Blombäck B, Blombäck M, Struwe I. Studies on blood clotting: factor VIII. Ark. for Kemi 15: 223 1961/1962
    PubMed
  • 5 van Creveld S, Pascha C. N, Veder H. A. The separation of A.H.F. from fibrinogen. Thrombos. Diathes. haemorrh. (Stuttg.) 15: 538 1966;
    PubMedSearch in Google Scholar
  • 6 Veder H. A. Further Purification of the Antihaemophilic Factor (A.H.F.). Thrombos. Diathes. haemorrh. (Stuttg.) 16: 738 1966;
    PubMedSearch in Google Scholar
  • 7 Wagner R. H, Lester W. D. M, Smith M, Brinkhous K. M. Purification of antihaemophilic Factor (Factor VIII) by amino acid precipitation. Thrombos. Diathes. haemorrh. (Stuttg.) 11: 64 1964;
    PubMedSearch in Google Scholar
  • 8 Wieme R. J. Studies on agar gel electrophoresis. Arscia uitgaven N.V.Brussel: 201 (1959).
    PubMed
  • 9 Hershgold E. J, Pool J. G, Pappenhagen A. R. The potent antihemophilic globulin concentrate derived from a cold insoluble fraction of human plasma : characterisation and further data on preparation and clinical trial. J. Lab. clin. Med. 67: 23 1966;
    PubMedSearch in Google Scholar
  • 10 Ratnoff O. D, Kass L, Lang P. D. Rapid purification of human antihaemophilic factor (factor VIII). J. Lab. clin. Med. 72: 1007 1968;
    PubMedSearch in Google Scholar
  • 11 Veltkamp J. J, Orion E. F, Loeliger E. A. Detection of the Carrier State in Hereditary Coagulation Disorders. Thrombos. Diathes. haemorrh. (Stuttg.) 19: 279 1968;
    PubMedSearch in Google Scholar
  • 12 Poison F. A. Fractination of protein mixtures on columns of granulated agar. Biochim. bio-phys. Acta (Amst.) 50: 565 1961;
    CrossrefPubMedSearch in Google Scholar
  • 13 Pool J. G, Shannon A. E. Production of high-potency concentrates of antihemophilic globulin in closed bagsystem : Assay in vitro and in vivo. New Engl. J. Med. 273: 1443 1965;
    CrossrefPubMedSearch in Google Scholar
  • 14 Wagner A. Über den diagnostischen Wert der Eiweiß-gebundenen Hexosen. Klin. Wschr. 23: 1187 1960;
    PubMedSearch in Google Scholar
  • 15 Elson L. A, Morgan W. T. J. A colormetric method for the determination of glucosamine and chondrosamine. Biochem. J. 27: 1824 1933;
    CrossrefPubMedSearch in Google Scholar
  • 16 Radhakrishnamurthy B, Berenson G. S. Differential determination of glucosamine and galac to samine. Clin. chim. Acta 10: 562 1964;
    CrossrefPubMedSearch in Google Scholar
  • 17 Böhm P, Dauber S, Baumeister L. Über Neuraminsäure, ihr Vorkommen und ihre Bedeutung im Serum. Klin. Wschr. 13: 289 1954;
    PubMedSearch in Google Scholar
  • 18 Kuntz E, Arnold G. Die klinische Aktivitäts-Beurteilung der Lungentuberkulose. S.58. Georg Thieme; Stuttgart: 1963
    Search in Google Scholar
  • 19 Dische Z. A new specific color reaction of hexuronic acids. J. biol. Chem. 167: 189 1947;
    PubMedSearch in Google Scholar