Subscribe to RSS
DOI: 10.1055/s-0038-1651426
Studies on Blood Coagulation Factor V
I. The Interaction of Salts of Fatty Acids and Coagulation FactorsPublication History
Publication Date:
10 June 2018 (online)
Summary
The interaction between stearates and coagulation factors consists of adsorption of the factors and partial activation resulting in thrombin formation. Factors V and XI are most readily adsorbed, closely followed by factor X.
Activation is seen most clearly with low concentrations of the adsorbant. It can be inhibited by omission of Ca++ ions and addition of hirudin, thus it involves most probably the generation of thrombin. No thrombin is found in the reaction mixture due to the strong adsorption of thrombin on stearates. The effects of activation are small compared to the effect of adsorption time, pH, temperature and hirudin.
Activity of factor V can be demonstrated in the adsorbed state but the factor cannot be eluted. Factor X shows no activity when adsorbed, but can be eluted from the powder. This suggests that the biological activity of factor V resides in a hydro-phylic part of the molecule, whereas the active centre of factor X probably is hidden but not destroyed by hydrophobic surfaces. The implications of these findings for the concept of prothrombinase formation on phospholipids are discussed.
-
References
- 1 Barton P. G, Jackson C, Hanahan D. J. Relationship between factor V and activated factor X in the generation of prothrombinase. Nature (Lond.) 214: 923 1967;
- 2 Glanss A. Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Actahaemat. (Basel) 17: 237 1957;
- 3 Esnouf M. P, Jobin F. Lipids in prothrombin conversion. Thromb. Diath. Haemorrh. suppl. 17: 103 1965;
- 4 Hemker H. C, Esnouf M. P, Hemker P. W, Swart A.C.W, Macfariane B. G. Formation of prothrombin converting activity. Nature (Lond.) 215: 248 1967; a
- 4a Hemker H. C, Kahn M. J. P. Reaction sequence of blood coagulation. Nature (Lond.) 215: 1201 1967; b
- 5 Jobin F. Ph. D. Thesis, Oxford (1966).
- 6 Kahn M. J. P, Hemker H. G. Interaction of hydrophobic surfaces and the blood coagulation promoting pro teins. F. E. B. S. Abstracts, Oslo A51: 13 1967;
- 7 Markward F. Versuche zur pharmakologischen Charakterisierung des Hirudins. Naunyn-Schmiedeberg’s Arch. exp. Path. Pharmak. 234: 516 1958;
- 8 Van der Meer J, Hemker H. C, Loeliger E. A. Pharmacological aspects of vitamin K1; a clinical study in man. F. K. Schattauer; Stuttgart: 1968
- 9 Nossel H. L. The contact phase of blood coagulation. Blackwell Scientific Publications; Oxford: 1964
- 10 Prydz H. Studies on proconvertin (factor VII) IV. The adsorption on barium sulphate. Scand. J. clin. Lab. Invest. 16: 409 1964; b
- 11 Veltkamp J. J, Orion E. F, Loeliger E. A. Detection of the carrier state in hereditary coagulation disorders I. Thrombos. Diathes. haemorrh. (Stuttg.) 19: 279 1968;
- 12 Voss D. Barium sulphate adsorption and elution of the “prothrombin complex” factors. Scand. J. clin. Lab. Invest. 17 Suppl.84: 119 1965;
- 13 Voss D. Das Verhalten von Prothrombin, Factor VII, IX und X an BaSO4 . Hoppe Seyler’s Z. physiol. Chem. 348: 1163 1967;
- 14 Vroman L. Surface contact and thromboplastin formation. Ph. D. Thesis, Utrecht (1958).
- 15 Vroman L. Effects of hydrophobic surfaces upon blood coagulation. Thrombos. Diathes. haemorrh. (Stuttg.) 10: 455 1964;
- 16 Vroman L, Adams A. L. Possible involvement of fibrinogen and proteolysis in surface activation; a study with the recording ellipsometer. Thrombos. Diathes. haemorrh. (Stuttg.) 18: 510 1967;
- 17 Zittle C. A. Adsorption studies of enzymes and other proteins. Adv. Enzym. 14: 391 1952;